Test 1- DNA Quantification

Question 1

DNA Concentration

Answer 1

(Absorbance at A260) x (50 micrograms/mL) x (Dilution factor) = concentration (micrograms/mL)

Question 2

Mass of DNA in a sample

Answer 2

(Concentration in micrograms/mL) x (Volume of resuspension buffer in mL) = mass of DNA in micrograms

Question 3

Percent of DNA in a sample

Answer 3

(Mass of DNA in micrograms)/(mass of tissue sample in micrograms) x 100 = % of DNA

Question 4

Protein Contamination Ratio (A234/A260)

Answer 4

A234/A260 > 0.5

Question 5

Protein Contamination Ratio (A260/A280)

Answer 5

• A260/A280 < 1.7 = contaminated

- A260/A280 > 1.7 = pure

Question 6

Dirty Cuvette

Answer 6

A320/A260 > 0.05 = dirty cuvette