Test 1.1 Flashcards
Salmonella General characteristics.
- G (-) Rod.
- Fac. Anaerobe.
- Non- motile.
- Selective enrichment needed ( Na- selenite, cysteine).
- T: 35- 27.
- pH 7 - 7.5.
- Opt H2O .99, but range.
Salmonella decomposition.
- Lactase, Urease, Sucrose, Indole, Vogues Proskauer -ve.
- Glucose +ve.
- H2S prod +ve.
Salmonella Resistance.
- Well on food surface 28 d in refrigeration.
- pH less resistant vs e. coli.
Salmonella Inactivation.
- Freezing = but doesn’t ensure total inactivation.
- Heat = 60 2- 6 min, 70 1 min.
- pH= can ø grow in pH 5.1.
- Low Aw = reduced growth.
Salmonella Illness.
- Incubation= up to 48hrs but usually within 16.
- CS: Abd pain, vomit, diarrhoea for about a wk, self limiting, low mt.
- Long term = Septicemia and reactive arthrities.
- Source - PO, shed through feces, contamianted meat, egg, dairy product.
E. coli General Characteristics.
- G (-) rod.
- Fac anaerobe.
- OKHF Ag.
- T 37 ( range).
- pH EHEC can grow down to 4.4 aW= .95.
E.coli Decomposition.
- lactose, Indole, Methyl red.
- VP, citrate.
E.coli Resistance.
Medium.
E.coli Inactivation.
Cook all parts to >70.
Campylobacter General Characteristics.
- Curved G (-) rod.
- Fastidious - blood agar.
- Microaerophilic 3.6% O2 + 10% CO2 needed for growth.
- Thermophilic - opt 42, min 30.
- Selective culture with vanco, poly + trimethprim.
Campylobacter Decomposition.
- Oxidative.
- Does ø split carbs, E demands met through utilisation of protein AA + organic acids.
Campylobacter Resistance.
- Weak poor survival in environment but likes humid temp.
- Likes meat/ hand surface.
Campylobacter Inactivation.
- Heat 55º for 5 mins.
Campylobacter CS.
- Most common bacterial infection in Hu.
- Contamination via milk, on meat surface.
- Incubation 1 - 10 D.
- M. pain, fever, diarrhea, nausea.
- Reiter Syn - autoimmune “ Can’t see, can’t pee, can’t climb a tree”.
- Guillian Barre Syn- acute polyneuropathy affecting PNS.
Listeria General cahracteristics.
- G+ rod, ciliated at 22ºC, can become + IC.
- Selective enrichment cold U tube.
Listeria Decomposition.
- B hemolysis, CAMP test, Rhamnose.
- Oxidase.
Listeria Resistance.
- High.
- pH = down to 4.4 need organic acid to kill.
- Fridge down to 0 º.
Listeria Inactivation.
- Boil 5 min at 60.
- Rapid pasteurization.
Listeria CS.
- Source is raw milk + milk products ( can survive ripening).
- Flu like but care in pregnant causes abortion, fetopathogen.
- CNS symptoms possible.
S. aureus General Characteristics.
- SPherical G +.
- Fac anaerobe.
- Mesophile.
- Halophile can tolerate <20% NACl.
- ø fastidious.
- Makes heat stable toxins, danger, MRSA.
S. aureus Decomposition.
- 30% are coagulase +ve.
S. aureus Resistance.
- Good, mnths in dry env.
S. aureus Inactivation.
UV light sterilization.
S. aureus CS.
- Contaminated meat + dairy products, ø kept hot/ cold enough.
- Acute symptoms in hrs.
- Diarrhea, vomit, pain, suppurative inflammations inanimals ( mastitis, sept, arthritis, derm), mastitis strains only rarely in milk.
- Animal strains do ø infect people + vice versa.
Botox General Characteristics.
- Anaerobic G
- Spore forming.
- Differentiated by toxins in humans A, B + E.
- Toxins cause the inhibition of neurotransmitter AcH release causing a flaccid paralysis.
Botox Resistance.
- Spore is very high, resistant to heat, radiation, ionisation.
- Veg are sensitive.
Botox Inactivation.
Heat to 80 for 10 mins or more.
Botox CS.
- Acute disease, death may be without signs due to paralysis of m. + heart.
- For animals carrion in stomach + hemorrhages in GI is suspect, condemnation of carcass.
C. Perfringens General Characteristics.
- G+ rod.
- Spore forming.
- A- E enterotoxins.
- Opt T 43- 47.
pH lowest is 5, opt 6 - 7.5.
C. perfringens resistance.
- Spore is very high
- Veg low, sensitive to acids + salts.
C. perfringens CS.
- Acute, from contaminated food esp meat, spores are in cont environment.
- Nausea, pain, H2Ory diarrhea, self- limiting ø tx needed.
C. perfringens inact/ prevention.
- Consume prepared foods quickly/ cool to < 10 within 2 - 3 hrs.
Bacillus Cereus.
- G+ rod.
- Spore forming.
B. cereus Inactivation.
- Diarrhea form: 55º for 5 min causes fever + H2Ory diarrhea.
- Emetic form: peptide toxin, inactivate by 126º for 90mins, binds to 5HT R leading to stim of vagus found in rice + pasta.