Practicals Flashcards
Define meat
The skeletal muscle and the closely attached edible tissues (fat, connective tissue, blood) of food producing animals or Every part of the animal’s body from which food can be produced
Organoleptic properties of meat
PIGS CRIBS Colour (scarlet red) pH Specific smell Glossy Inelastic Incomplete bleeding Interwoven w CT and fat Body condition Rot Stuffiness
Influences of organoleptic properties of meat
Maturation Species, breed, age, gender, body part Feed Slaughtering process
pH change in meat caused by
Glycogen (pH 7.6) b/d to Lactic Acid (pH 5.5)
Measuring pH
Nitrazine yellow Bromothymol blue Electric pH meter Indicator paper
Colour of myoglobin
Purple red
Colour of oxymyoglobin
scarlet red
Colou of methimoglobin
brown red
Colour of sulfmyoglobin
yellow
Colour of cholemyoglobin
green
Colour with high O2
oxymyoglobin
Colour with low O2
metmyoglobin
Colour with no O2
deoxymyoglobin
Yellow coloured meat caused by
– Lipochromatosis (Carotenoids +) – Lipofuscin-like pigments (Unsaturated fatty acids +) – Icterus / Jaundice: Pathological
Black coloured meat caused by
Melanin (lungs), porphyrins (ribs)
Yellow discolouration examination
Alcohol ether -Jaundice: BR dissolves in alcohol Carotenoids: dissolves both in ether
Test smell
Organoleptic judgement- boiling and roasting
Abnormal smell
• Alimentary (e.g. fishmeal, moldy feed) • Boar taint (androstenone, skatole) • Antemortem (disease, metabolic disorder – Lab examination) • Postmortem (acquired from the environment)
Incomplete bleeding results in
hemoglobin infiltration of the meat
Reasong for incomplete bleeding
– Slaughtering technology – Disorder of the circulatory / nervous system – Toxicosis – Infection – Stress
Determination of incomplete bleeding
Reder test (Löffler reagent – Methylene blue)
Rot: cause of it?
Bacterial decomposition of nitrogenous compounds
Rot effect on pH
increases it
Rot effect on protein
biogenic amines, indole, skatole, H2S, NH3, CO2
Rot effect on fat
volatile fatty acids, palmitic acid
Detection of rot
NH3 presence (Eber’s test, Nessler’s test)
Rot: microorganisms
PAM CAP Pseudomonas, Proteus, Achromobacter, Micrococcus, Alcaligenes spp. Anareobic: deep inside the large muscles Clostridium spp. > Gas production
Stuffiness
Enzimatic degradation of nitrogenous compounds Hydrogen-sulfide (H2S) production
Stuffiness effect on pH
Acidic / neutral pH
Detection of stuffiness
Lead acetate/Lead nitrate test
Consistency: compressibility
Grau & Hamm: filter paper press method
Tenderness, texture
– Sensory judgment – Enzymatic digestion – Warner-Bratzler shear force test
Liquid-holding capacity
• Proteins: actin, myosin, sarcoplasma proteins • Dissolvedsalts • pH
Liquid holding capacity: determination
– Grau & Hamm method: 0,3g meat + filter paper (5 mins) – Liquid leakage method: measuring drip loss – Drying method
Experiments: pH
indicator paper
Experiments: cooking test
Bunsen burner, pot
Experiments: Reder’s test
Loeffler reagent (methylene blue)
Experiments: Eber’s test
glass tube, NH3
Experiments: Nessler test
Nessler’s reagent, NH3
Experiments: Liquid holding capacity
Grau-Hamm
Experiments: Consistence
Grau-Hamm filter-paper press method
Reg. No. 852/2004/EC
hygiene of foodstuffs
853/2004/EC
hygiene rules for food of animal origin
854/2004/EC
official controls on products of animal origin intended for human consumption
Normal, routine slaughter
individual or group slaughter of healthy animals
Separate slaughter
suspected infection or disease; separated in terms of space and time
Emergency slaughter
urgent bleeding of any injured but healthy animal (e.g. after accident)
ANTE MORTEM I
Inspection of the animals in the farm
ANTE MORTEM II
Inspection of the animals in the slaughterhouse
POST MORTEM
Meat inspection after slaughtering
Integrative meat inspection
Ante-mortem 1 & 2 PM Hygiene during slaughtering Lab testing (bact, paras, chem)
Tasks of the vet
Documentation (AM 1) Inspect vehicle (AM 2) Doc. check, decisions re animals (AM 2) Health mark, trichinella test (PM)
AM inspection: documentation at the farm
Health certificate Food Chain Information Animal Passports (ENAR)
FCI gives information about
- the farm - the occurrence of diseases that may affect the meat safety, - the health status of the animals (in the herd of origin and at the region level), - the medical or other treatments, - previous meat inspection reports (repeatedly high frequency of pathological lesions) - monitoring results (zoonoses and residues)
Control of documentation at the slaughterhouse
- Health certificates 2. Food chain information 3. Animal passports 4. Documentation of the transport vehicle (randomly)
Ante mortem II. inspection
- check for symptoms and lesions: contagious or zoonotic animal diseases, wounds, infections… 2. animal welfare 3. food hygiene factors (cleanliness) 4. signs of given medication - monitor animals in place and in movement (lameness)
Decisions concerning live animals
After the control of the documentation and the ante mortem examination the state vet can 1. Permit routine, unconditional slaughter 2. Permit the slaughter with the detainment of the meat, max. 24 hours (if the documentation is incomplete) 3. Ban the slaughter until further measure 4. Order separate slaughter and detained storage 5. Order separate killing (no bleeding)
Post mortem inspection: Aim
- find any pathological sign (emaciation, oedema, colour changes, abscesses, bruises…) - provide information for the judgment of the meat. - determine acute/chronic, local/gener, prim/seco
PM techniques
Visual Palp Incision (risk of cross contamination) Olfaction Trichinella spp detection
Detection of Trichinella
- direct detection of first-stage larvae in striated muscle using tissue digestion. - pooled sample: positive or uncertain result→ a large sample is taken from each animal and tested in smaller batches until an individual positive animal is identified - Cannot leave the carcass until negative result
Health mark
FFHC Traceability (slaughterhouse) Multiple stamps (multiple pieces of carcass) Poultry: apply to packaging
By-products
SRM’s, TSE materials, parts of carcasses Inedible Manure Wastewater
The skeletal muscle and the closely attached edible tissues (fat, connective tissue, blood) of food producing animalsorEvery part of the animal’s body from which food can be produced
Define meat
PIGS CRIBSColour (scarlet red)pHSpecific smellGlossyInelasticIncomplete bleedingInterwoven w CT and fatBody conditionRot Stuffiness
Organoleptic properties of meat
MaturationSpecies, breed, age, gender, body partFeedSlaughtering process
Influences of organoleptic properties of meat
Glycogen (pH 7.6) b/d to Lactic Acid (pH 5.5)
pH change in meat caused by
Nitrazine yellowBromothymol blueElectric pH meterIndicator paper
Measuring pH
Purple red
Colour of myoglobin
scarlet red
Colour of oxymyoglobin
brown red
Colou of methimoglobin
yellow
Colour of sulfmyoglobin
green
Colour of cholemyoglobin
oxymyoglobin
Colour with high O2
metmyoglobin
Colour with low O2
deoxymyoglobin
Colour with no O2
– Lipochromatosis (Carotenoids +)– Lipofuscin-like pigments (Unsaturated fatty acids +) – Icterus / Jaundice: Pathological
Yellow coloured meat caused by
Melanin (lungs), porphyrins (ribs)
Black coloured meat caused by
Organoleptic judgement- boiling and roasting
Test smell
• Alimentary (e.g. fishmeal, moldy feed)• Boar taint (androstenone, skatole)• Antemortem (disease, metabolic disorder – Lab examination)• Postmortem (acquired from the environment)
Abnormal smell
hemoglobin infiltration of the meat
Incomplete bleeding results in
– Slaughtering technology– Disorder of the circulatory / nervous system – Toxicosis– Infection– Stress
Reasong for incomplete bleeding
Reder test (Löffler reagent – Methylene blue)
Determination of incomplete bleeding
Bacterial decomposition of nitrogenous compounds
Rot: cause of it?
increases it
Rot effect on pH
biogenic amines, indole, skatole, H2S, NH3, CO2
Rot effect on protein
volatile fatty acids, palmitic acid
Rot effect on fat
NH3 presence (Eber’s test, Nessler’s test)
Detection of rot
PAM CAPPseudomonas, Proteus, Achromobacter, Micrococcus, Alcaligenes spp.Anareobic: deep inside the large muscles Clostridium spp. > Gas production
Rot: microorganisms
Enzimatic degradation of nitrogenous compoundsHydrogen-sulfide (H2S) production
Stuffiness
Acidic / neutral pH
Stuffiness effect on pH
Lead acetate/Lead nitrate test
Detection of stuffiness
Grau & Hamm: filter paper press method
Consistency: compressibility
– Sensory judgment– Enzymatic digestion– Warner-Bratzler shear force test
Tenderness, texture
• Proteins: actin, myosin, sarcoplasma proteins • Dissolvedsalts• pH
Liquid-holding capacity
– Grau & Hamm method: 0,3g meat + filter paper (5 mins)– Liquid leakage method: measuring drip loss – Drying method
Liquid holding capacity: determination
indicator paper
Experiments: pH
Bunsen burner, pot
Experiments: cooking test
Loeffler reagent (methylene blue)
Experiments: Reders test
glass tube, NH3
Experiments: Ebers test
Nessler’s reagent, NH3
Experiments: Nessler test
Grau-Hamm
Experiments: Liquid holding capacity
Grau-Hamm filter-paper press method
Experiments: Consistence
hygiene of foodstuffs
Reg. No. 852/2004/EC
hygiene rules for food of animal origin
853/2004/EC
official controls on products of animal origin intended for human consumption
854/2004/EC
individual or group slaughter of healthy animals
Normal, routine slaughter
suspected infection or disease; separated in terms of space and time
Separate slaughter
urgent bleeding of any injured but healthy animal (e.g. after accident)
Emergency slaughter
Inspection of the animals in the farm
ANTE MORTEM I
Inspection of the animals in the slaughterhouse
ANTE MORTEM II
Meat inspection after slaughtering
POST MORTEM
Ante-mortem 1 & 2PMHygiene during slaughteringLab testing (bact, paras, chem)
Integrative meat inspection
Documentation (AM 1)Inspect vehicle (AM 2)Doc. check, decisions re animals (AM 2)Health mark, trichinella test (PM)
Tasks of the vet
Health certificateFood Chain InformationAnimal Passports (ENAR)
AM inspection: documentation at the farm
- the farm - the occurrence of diseases that may affect the meat safety, - the health status of the animals (in the herd of origin and at the region level), - the medical or other treatments, - previous meat inspection reports (repeatedly high frequency of pathological lesions) - monitoring results (zoonoses and residues)
FCI gives information about
- Health certificates2. Food chain information3. Animal passports4. Documentation of the transport vehicle (randomly)
Control of documentation at the slaughterhouse
- check for symptoms and lesions: contagious or zoonotic animal diseases, wounds, infections… 2. animal welfare 3. food hygiene factors (cleanliness) 4. signs of given medication - monitor animals in place and in movement (lameness)
Ante mortem II. inspection
After the control of the documentation and the ante mortem examination the state vet can1. Permit routine, unconditional slaughter2. Permit the slaughter with the detainment of the meat, max. 24 hours (if the documentation is incomplete)3. Ban the slaughter until further measure4. Order separate slaughter and detained storage5. Order separate killing (no bleeding)
Decisions concerning live animals
- find any pathological sign (emaciation, oedema, colour changes, abscesses, bruises…) - provide information for the judgment of the meat. - determine acute/chronic, local/gener, prim/seco
Post mortem inspection: Aim
VisualPalpIncision (risk of cross contamination)OlfactionTrichinella spp detection
PM techniques
- direct detection of first-stage larvae in striated muscle using tissue digestion. - pooled sample: positive or uncertain result→ a large sample is taken from each animal and tested in smaller batches until an individual positive animal is identified - Cannot leave the carcass until negative result
Detection of Trichinella
FFHCTraceability (slaughterhouse)Multiple stamps (multiple pieces of carcass)Poultry: apply to packaging
Health mark
SRM’s, TSE materials, parts of carcassesInedibleManureWastewater
By-products