Test 1 material

Question 1

In prokaryotes the DNA forms a tangle known as a

Answer 1

nucleoid

Question 2

all bacteria have a cell wall. T/F?

Answer 2

TRUE

Question 3

in the DNA chain, phsophodioester bonds join each deoxynucleotide T/F?

Answer 3

TRUE

Question 4

in the double helix of DNA, the DNA chains are joined by

Answer 4

hydrogen bonds

Question 5

Meselson and Stahl’s experiment proved replication of DNA to be

Answer 5

semiconservative replication

Question 6

The DNA polymerase I makes the Okazaki fragments T/F

Answer 6

FALSE

Question 7

The replication in the leading strand is

Answer 7

continuous and 5-3

Question 8

In bacteria the replication is unidirectional T/F

Answer 8

FALSE

Question 9

In bacteria there are several origins of replication T/F

Answer 9

false

Question 10

Replication continues until the replication fork enters a region of the chromosome called Ter T/F?

Answer 10

TRUE

Question 11

The RNA polymerase makes a complementary copy of the DNA using the strand 5-3 t /f

Answer 11

false

Question 12

The tertiary structure of RNA is called

Answer 12

pseudonot

Question 13

The promoter is recognized by the sub-unit alpha of the RNA polymerase
t/f

Answer 13

FALSE

Question 14

In prokaryotes different types of RNA Polymerase synthesizes mRNA, tRNA and rRNA
t/f

Answer 14

false

Question 15

In the transcription the process in which the RNA polymerase recognizes and binds to a promoter sequence on DNA is called

Answer 15

initiation

Question 16

A sequence of three nucleotides in mRNA that specifies the first amino acid in the synthesis of a polypeptide chain

Answer 16

start codon

Question 17

What amino acid sequence would the DNA base sequence 5’ CGCATGAAAGAGCCT 3’ code for?

Answer 17

Arg-Met-Lys-Glu-Pro

Question 18

A complex consisting of a 30S ribosomal subunit, a tRNA having the anticodon UAC and carrying an altered form of the amino acid methionine (N-formylmethionine or f-Met), and proteins called initiation factors

Answer 18

initiation complex

Question 19

The ribosomal subunit that binds to mRNA to form the initiation complex.

Answer 19

30S ribosomal subunit

Question 20

The ribosomal site where an aminoacyl-tRNA first attaches during translation.

Answer 20

A-site of ribosome

Question 21

The ribosomal site where the growing amino acid chain is temporarily being held by a
tRNA as the next codon in the mRNA is being read.

Answer 21

P-site of ribosome

Question 22

Bacteria that cannot make or lacks the ability to grow on a particular nutrient.

Answer 22

Auxotroph

Question 23

Newcomb experiment proof the direct mutation hypothesis

t/f

Answer 23

false

Question 24

Mutations are permanent changes in the DNA sequence that are not inherited
t/f

Answer 24

false

Question 25

Transitions, transversions, missense mutations and Nonsense mutations are included in the

Answer 25

base pair changes

Question 26

Inversion is a mutation cause by cause by recombination between repeats and it can revert
t/f

Answer 26

false

Question 27

Suppression: alleviation of the effects of a mutation by second mutation elsewhere in the DNA
t/f

Answer 27

true

Question 28

Cis acting mutation affect a functional region on the DNA molecule that does encode a gene product
t/f

Answer 28

false

Question 29

Procedure where using molecular genetic techniques in which a cloned gene is altered in the test tube and then reintroduced into the organism, then the altered gene replace the corresponding normal gene

Answer 29

gene replacement

Question 30

If a DNA strand reads 3’-TACGGCATG (where the names of the bases are abbreviated), then the RNA molecule created by transcription would read

Answer 30

AUGCCGUAC

Question 31

In the bacterial chromosome replication the DnaA multimer complexes with ATP causes melting of an AT rich region to which another protein, DnaB/DnaC/ATP complex can then bind.
t/f

Answer 31

true