Test 1 material Flashcards
In prokaryotes the DNA forms a tangle known as a
nucleoid
all bacteria have a cell wall. T/F?
TRUE
in the DNA chain, phsophodioester bonds join each deoxynucleotide T/F?
TRUE
in the double helix of DNA, the DNA chains are joined by
hydrogen bonds
Meselson and Stahl’s experiment proved replication of DNA to be
semiconservative replication
The DNA polymerase I makes the Okazaki fragments T/F
FALSE
The replication in the leading strand is
continuous and 5-3
In bacteria the replication is unidirectional T/F
FALSE
In bacteria there are several origins of replication T/F
false
Replication continues until the replication fork enters a region of the chromosome called Ter T/F?
TRUE
The RNA polymerase makes a complementary copy of the DNA using the strand 5-3 t /f
false
The tertiary structure of RNA is called
pseudonot
The promoter is recognized by the sub-unit alpha of the RNA polymerase
t/f
FALSE
In prokaryotes different types of RNA Polymerase synthesizes mRNA, tRNA and rRNA
t/f
false
In the transcription the process in which the RNA polymerase recognizes and binds to a promoter sequence on DNA is called
initiation
A sequence of three nucleotides in mRNA that specifies the first amino acid in the synthesis of a polypeptide chain
start codon
What amino acid sequence would the DNA base sequence 5’ CGCATGAAAGAGCCT 3’ code for?
Arg-Met-Lys-Glu-Pro
A complex consisting of a 30S ribosomal subunit, a tRNA having the anticodon UAC and carrying an altered form of the amino acid methionine (N-formylmethionine or f-Met), and proteins called initiation factors
initiation complex
The ribosomal subunit that binds to mRNA to form the initiation complex.
30S ribosomal subunit
The ribosomal site where an aminoacyl-tRNA first attaches during translation.
A-site of ribosome
The ribosomal site where the growing amino acid chain is temporarily being held by a
tRNA as the next codon in the mRNA is being read.
P-site of ribosome
Bacteria that cannot make or lacks the ability to grow on a particular nutrient.
Auxotroph
Newcomb experiment proof the direct mutation hypothesis
t/f
false
Mutations are permanent changes in the DNA sequence that are not inherited
t/f
false
Transitions, transversions, missense mutations and Nonsense mutations are included in the
base pair changes
Inversion is a mutation cause by cause by recombination between repeats and it can revert
t/f
false
Suppression: alleviation of the effects of a mutation by second mutation elsewhere in the DNA
t/f
true
Cis acting mutation affect a functional region on the DNA molecule that does encode a gene product
t/f
false
Procedure where using molecular genetic techniques in which a cloned gene is altered in the test tube and then reintroduced into the organism, then the altered gene replace the corresponding normal gene
gene replacement
If a DNA strand reads 3’-TACGGCATG (where the names of the bases are abbreviated), then the RNA molecule created by transcription would read
AUGCCGUAC
In the bacterial chromosome replication the DnaA multimer complexes with ATP causes melting of an AT rich region to which another protein, DnaB/DnaC/ATP complex can then bind.
t/f
true
