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PMG final > Quiz 2 and Quiz 3 > Flashcards

Quiz 2 and Quiz 3 Flashcards

1
Q

A replicon is

A

a DNA molecule that is able to replicate and be maintained

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2
Q

Plasmids can best be described as:

A

small circular DNA molecules that can exist independently of chromosomes commonly found in bacteria

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3
Q

In insertional activation

A

If foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies remain white

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4
Q

If host cells are ampicillin sensitive and are plated on a medium containing ampicillin:

A

only cells that have taken up the ampicillin resistance vector will grow

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5
Q

When foreign DNA and plasmid are both cut with the same restriction enzyme and mixed together, all molecules will form recombinants. T/F?

A

FALSE

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6
Q

theta replication is…

A

unidirectional and bidirectional

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7
Q

CoE1 plasmid do not require a plasmid encoded protein to initiate replication at their OriV, only a primer synthesized from the plasmid. T/F?

A

TRUE

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8
Q

the iteron plasmids only use host proteins in the replication T/F?

A

FALSE (they use plasmid encoded RepA)

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9
Q

Plasmids give bacteria a selective advantage under certain conditions. T/F?

A

TRUE

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10
Q

The plasmid members of the same incompatibility group are able to coexist/ T/F?

A

FALSE (they cannot coexist)

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11
Q

Curing a plasmid is when bacteria lose the plasmid during cell division T/F?

A

TRUE

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12
Q

ColE1 derived plasmids replication is initiated when RNA II form a hybrid DNA-RNA at oriV region. T/F?

A

TRUE

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13
Q

The OriV region in the plasmid determines the bacteria host, regulates, the number of copies, but not the mechanism of replication. T/F?

A

FALSE (it also determines the mechanism of replication)

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14
Q

In the rolling circle replication, the plasmid have a single strand origin and double strand origin regions. T/F?

A

TRUE

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15
Q

Most of the plasmids require a plasmid encoded protein called _______.

A

Rep

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16
Q

During the course of an E coli transformation laboratory, a student forgot to mark the culture tube that received the kanamycin resistant plasmids. The student proceeds with the laboratory because he thinks that he will be able to determine from his results which culture tube contained cells that may have undergone transformation. Which plate would be most likely to indicate transformed cells?

A

A plate with 100 colonies growing on LB agar with kanamycin.

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17
Q

The F plasmid codes for…

A

making the F pilus

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18
Q

The F pilus is…

A

a protein appendage on the F+ donor that attaches to specific receptors on the cells wall of a recipient.

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19
Q

When an F+ donor gives an F plasmid to an F- recipient…

A

both become F+

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20
Q

Contact is required between an F+ and an F- cell for conjugation to occur, T/F?

A

A

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21
Q

When F+ cells are mixed with F- cells, eventually all cells become F+. T/f?

A

TRUE

22
Q

DNA transformation involves the transfer of DNA via…

A

naked DNA in solution

23
Q

The mismatch repair system will repair

A

either the donor or recipient strand

24
Q

When DNA from an ampicillin resistant organism is brought into an ampicillin sensitive organism by transformation and the product is grown on agar containing ampicillin…

A

only the transformed cells will grow

25
Q

The relationship between a virus and host where no new viral particles are produced and the viral genome is replicated along with the host chromosome is called…

A

lysogeny

26
Q

In which type of transduction does the transducing particle carry only specific portions of the bacterial genome?

A

specialized transduction

27
Q

which of the following are true un regards to F+ x F- mating events?
A. DNA is transferred from F+ to F- cells
B. DNA is transferred from F- to F+ cells.
C. No DNA is transferred because F- cells are unable to perform conjugation.

A

A. DNA is transferred from F+ to F- cells.

28
Q

this type of plasmid can exist with or without being integrated into the host chromosome.

A

Episome

29
Q

In the lytic cycle, the bacterial cell is ruptured by the action of?

A

Lysozyme.

30
Q

Predict the phenotype of a lacI mutant

A

The lac genes would be expressed continuously.

31
Q

If a second wild type or normal copy of the lacI gene is introduced into the lacI mutant cell, what would be the phenotype of this partial diploid. (merodiploid)

A

The lac genes would be expressed efficiently only in the presence of lactose.

32
Q

Predict the phenotype of a lacIs or a super repressor mutant. A lacIs mutant synthesizes a repressor that cannot bind to the inducer.

A

The lac genes would never be expressed efficiently.

33
Q

Predict how a lacIs mutant would be affected by the construction of a merodiploid that has a second normal copy of the lacI gene.

A

The lac genes would never be expressed efficiently.

34
Q

Predict the phenotype of an operator mutant Oc which would prevent the binding of the repressor.

A

the lac genes would be expressed continuously.

35
Q

Predict the phenotype of a promoter mutant lacP which has a mutation in the promoter for the lac operon.

A

The lac genes would never be expressed efficiently.

36
Q

Which of the following does not occur in the function of the catabolite activator protein of e coli?
A. cyclic AMP binds to the CAP protein
B. the protein changes shape
C. space is increased by the binding of tryptophan
D. helix turn helix motifs are enabled to bind to DNA

A

C. space is increased by the binding of tryptophan

37
Q

When tryptophan is present in the environment of e coli the tryptophan binds to the

A

trp promoter

38
Q

in order for a gene to be transcribed RNA polymerase must have access to the DNA helix and be able to bind to the genes.

A

promoter

39
Q

e coli is able to use foods other than glucose in the absence of available glucose, because falling levels of glucose cause an increase of?

A

cAMP

40
Q

In the absence of glucose, e coli can import lactose to change into glucose and galactose because CAP binds to the…

A

DNA

41
Q

which type of growth media would you expect to result in synthesis of high levels of mRNA for the enzymes of the e coli lac operon?

A

no glucose, high lactose

42
Q

lac operon has how many operators?

A

3

43
Q

the arabinose operon uses positive regulation/. T/F?

A

FALSE (it uses both pos and neg)

44
Q

AraC turns off transcription of the ara operon by binding to the araI initiator site only when it is bound by arabinose. T/F?

A

FALSE (it turns it on)

45
Q

the regulatory protein araC can act as both a repressor and an activator. T/F?

A

TRUE

46
Q

Single CAP binding site required for the activation of the AraBAD promoter for the araC promoter. T/F?

A

TRUE

47
Q

manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. What is the circular piece of DNA called?

A

plasmid

48
Q

manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. What tool is used to cut the gene?

A

restriction enzyme

49
Q

genetic engineering manipulates gene products at the level of…

A

DNA

50
Q

An E. coli strain is F- lac Z- met+ bio+. Cells from this strain are mixed with an E. coli strain that is lac Z+ met- bio- and carrying an F’ episome with the plac O+ lac Z+ DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become lac Z+ met+ bio+?

A

sexduction, a special type of conjugation

51
Q

How many lac Z genes were present in the cells described in problem 3 that were able to grow on minimal media?

A

2, the lacZ- allele on the bacterial chromosome and the lacZ+ allele on the F’ episome

52
Q

Regulation of lac Z gene on an F’ episome. How would you describe the regulation of lactose metabolism in the cells described in problem 3 that were able to grow on minimal media with lactose as a nutrient?

A

normal regulation of lactose metabolism

PMG final (4 decks)

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