PCR questionnaire

Question 1

Who received the Nobel Prize in chemistry in 1993 for inventing PCR?

Answer 1

Kary Mullis

Question 2

PCR stands for?

Answer 2

polymerase chain reaction

Question 3

three steps of PCR?

Answer 3

1. Denature DNA
2. Anneal primers
3. Extend DNA

Question 4

When DNA copies are multiplied at a rapid rate, this is referred to as ______ amplification.

Answer 4

exponential

Question 5

The DNA or RNA used in a PCR reaction is called the?

Answer 5

template

Question 6

during the annealing step…

Answer 6

primers bind to the newly separated DNA strand

Question 7

nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the ____ phase.

Answer 7

extension/ elongation

Question 8

Which of the following is not true about Taq DNA polymerase?
A. it functions at extremely high temperatures
B. it adds nucleotides triphosphates to form a new DNA strand
C. it requires a salt environment (buffer) to work
D. It can act as an en exonuclease to cut single stranded RNA

Answer 8

D. It can act as an en exonuclease to cut single stranded RNA

Question 9

what type of PCR quantifies the rate at which new DNA is synthesized?

Answer 9

Real Time

Question 10

What is a hot start?

Answer 10

When a high temperature is maintained before polymerase is added
It reduces non specific primer binding