Test 1(CH3) Flashcards

1
Q

two most common shapes of Bacteria

A

Cocci and rods
-cocci are circles
-rods are like small spaghettis

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2
Q

Cell wall of gram-positive bacteria is consist of a single ________ layer of _____

A

20-80 nm thick layer of peptidoglycan (murein)

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3
Q

Cell wall of a gram-negative bacteria has 2 layers: _____ & ________

A

2-7nm peptidoglycan layer & covered by 12-14 nm thick outer membrane

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4
Q

space between plasma membrane and the outer membrane is called ______

A

periplasmic space

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5
Q

Feature of all bacterial cells on their cell wall is _________, also called as _________

A

peptidoglycan , as peptidoglycan sacculus

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6
Q

2 different cross-links structure of a petidoglycan sacculus

A

Direct & indirect

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7
Q

Direct crossed0link P sacculus is _____

A

connection of a carboxyl group of an amino acid in one stem petide to the amino group of an amino acid in another stem peptide

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8
Q

Most bacteria that stain gram-positive belong to only 2 phyla, _____ & ______

A

Antinobacteria & firmicutes

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9
Q

Most of gram positive’s cell wall bacteria are composed of

A

Peptidoglycan and teichoic acids

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10
Q

Teichoic acids functions:

A

help creat & maintain the structure of the cell envelope by “anchoring the wall to the plasma membrane “

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11
Q

gram negative cells walls on a bacteria has additional layers (Tor F)

A

T since the peptidoglycan on negative cell wall are very thin because it is also contains an outer membrane. (this outer membrane is also special because _______)

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12
Q

the structure of the outer membrane of the the gram negative is special because

A

the external layer (facing the environment) is comprised of LIPOPOLYSACCHARIDES. while the inner layer (facing the cell) contains phospholipids

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13
Q

Lipopolysaccharides’ (LPS) structure contains Lipid A , inner core, outer core, O-antigen

A

Lipid A contains 2 glucosamine sugar derivatives (with fatty acids & phosphate attached). Lipid A is responsible for the hydrophobic component

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14
Q

LPS Lipopolysaccharides function

A

(1) contributes to the negative charge on bacteria’s surface since the core polysaccharide contains charged sugars and phosphate
(2)stabilize lipid A
(3) helps create a permeability barrier
(4) protects from pathogenic bacteria (because of O-antigen)
(5) Lipid A portion of LPS can act as a toxin and is called Endotoxin

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15
Q

A class of protein called ______ that helps move solutes through the pol outermsmbrane of a negative gram cell wall is

A

is Porin

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16
Q

A process called plasmolysis is

A

bacteria is in hypertonic solution (less water outside cell) water flows out and cytoplasms shrivels up.

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17
Q

If bacteria is in hypertonic soulution

A

Plasmolysis process will occur and cytoplasm chrivels

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18
Q

Lysis is the process

A

when cel membrane breaks from having too much water going in

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19
Q

Treatment of a typical gram positive bacteria with _____ & _____ will result into a complete loss of cell wall. the cell wall will become _______

A

Lysosome & penicillin. it will become protoplast.

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20
Q

Treatment on a negative gram bacteria with ____ & ______. the Peptidoglycan sacculus will be destroyed and will be called _______

A

Lysosome or penicillin. and will become Spheroplast.

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21
Q

Extracellular Vesicle function (that tiny bulge or storage like on the side of the bacteria)

A

Transfer toxin molecules during infection, transfer genetic material between cells. they carry some ATP

22
Q

Bacteria has a surface covering called ______ , & what is it made of ?

A

S- Slayer, made of protein like glycoprotein.

23
Q

S- layers has some other functions like

A

Protecting the cell against ion & pH fluctuations, osmotic stress, enzymes, or predatory bacteria
It also gives adhesion to surfaces and maintain shape.

24
Q

Why are S-layers important in nanotechnology?

A

it is because of their ability to self-assemble into an S-layer without any aid.

25
Protoplast is ________ and _____
The plasma membrane and everything inside it
26
Pilis are responsible for attachment into another cell or to solid surfaces & can only be seen by
Electron microscope
27
Type IV pili are involved in _______
motility and two gene transfer mechanism: bacterials transformation & bacterial conjugation
28
Bacteria has around ____ sex pili. They are ___ to other pili
10 sex pilis, larger to other pilis
29
Bacterial Flagella function
Motility
30
Monotrichous bacteria has
only 1 fragellum
31
Amphitrichous has
2 flagellum on both side
32
Lophotrichous bacteria
has a cluster of flagellum on either side of the bacteria
33
Bacteria flagellum is composed of 3 parts :
(1) filament (longest portion) (2)basal body embedded in the cell envelope
34
Bacteria flagellum is composed of 3 parts :
(1) filament (longest portion) (2)basal body embedded in the cell envelope (3) hook- link the filament to its basal body and act as a flexible coupling
35
Gram negative bacteria have _ rings connected to a central rod of the flagellum
4 rings : L, P, MS, and C. the L, P, MS are embedded in the lipopolysaccharide, peptidoglycan, and plasma membrane, respectively and the C ring on the cytoplasmic side of the MS ring
36
Gram positive bacteria only have 2 rings
inner ring connected to the plasma membrane, and outer one attached to the peptidoglycan
37
How does filament grow as a flagellum grow on it's normal size,
it starts growing from the base and then grows more on the very tip. filament synthesis are self-assembly
38
Bacteria's movement toward or away chemical stuff is known as
chemotaxis
39
Motile bacteria move in response to environmental cues such as temperature ______, light _____, oxygen ______, osmotic ______ , and magnetic field ________
thermotaxis, phototaxis, aurotaxis, osmotaxis, magnetotaxis
40
how does bacteria move its flagellum to move
counter clockwise to run forward & clockwise to tumble
41
Endospore
dormant cells formed within mother cells, and produced by the phylum Firmicutes
42
What is sporulation
is when bacterial growth slows down due to nutrient limitation (common on soil bacteria)
43
2 main size of bacteria
Cocci, rods
44
less commons ones are
vibrios (comma shaped), spirilla ( rigid spiral-shaped bacteria), spirochetes ( flexible &spiral-shaped bacteria), pleomorphic (lacking a characteristic form)
45
Actinobacteria form long filaments called _____ and then this form a network called a
hyphae , mycelium
46
Why are bacteria so small?
Because o greater surface area to volume ratio
47
what do you call around the cell membrane, capsule, and cell wall of the bacteria?
cell envelope
48
extracellular layers of a bacterial cell according to their relationships. (Capsule - slime layer, S-Layer )
Capsule organize sugar level , Slime layer disorganize sugar level, and S-Layer organize protein layers
49
A bacteria that has single flagella
Monotrichious
50
2 flagellas at the opposing side
Amphitrichous
51
A bunch of flagellas on either end of the bacteria
Liphotrichous
52
Bacteria that has ba bunch of flagellas all over their cell membrane
peritrichous