Techniques of Microscopy Flashcards
What is acidophilic staining?
Tissue components that stain with acid dyes
What is Eosin?
An acid dye, pink-orange color used in acidophilic staining.
What structures are stained by eosin?
General cytoplasm, mitochondria, secretory and lysosomal granules and red blood cells
Whats structures are stained my hematoxylin?
Organelles with substantial amount of DNA and RNA.
True or False. The affinity of the dyes to tissue components and staining has no relation to the biochemistry of the components in the LIVING state.
True
What dyes are tissues routinely stained with?
Hematoxylin and Eosin
Which three cell structures are commonly Acidophilic staining?
Mitochondria, Secretory vesicles, and Lysosomes
Which two cytoplasmic structures will result in Basophilic staining?
Ribosomes and Secretory vesicles
Which cytoplasmic materials result in empty or clear staining?
Lipids, Glycogen, and Mucus
