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Molecular Genetics > Techniques Based On Hybridisation > Flashcards

Techniques Based On Hybridisation Flashcards

1
Q

Microarray

A

Denature labelled probe
Denature chromosome on fixed slide
Hybridisation occurs

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2
Q

PCR

A 
Denaturation 
Annealing (primer binding) select portion of genome for amplification
Elongation (DNA polymerase)
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3
Q

Example of a technique

A 
Northern blotting (DNA) negatively charged 
Migrate due to size
Nitrocellulose filter
Probes (fluorescent/radioactive)
Visualise
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Molecular Genetics (10 decks)

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