Lecture-1

Question 1

What are the two forms of chromatin?

Answer 1

Heterochromatin (condensed, transcriptional inactive, often ATs) and Euchromatin (unravelled, can be transcribed, contains coding regions)

Question 2

What is chromatin

Answer 2

DNA wound round histone proteins

Question 3

Giemsa: G banding

Answer 3

Chromosomes treated with trypsin to digest proteins, then stained with giemsa dye that binds phosphate groups. Dark bands form with more AT pairs (heterochromatin)