Technical skills and applications Flashcards
1
Q
Why is plasmid DNA is purified using anion exchange column chromatography?
A
DNA is negatively charged, binds to the positively charged resin, and is washed out with increasing salt.
2
Q
In recombinant DNA technology, DNA ligase is used to rejoin which of the following bonds in DNA?
A
Phosphodiester
3
Q
Sandwich ELISAs are commonly used in commercial diagnostic kits because of the increased accuracy of the assay. Which of the following contributes to the increased level of accuracy?
A
The antigen must be recognized by two primary antibodies for the assay to give a positive result.
4
Q
What is 2.420 x 0.065, rounded to the correct number of significant digits?
A
.16
