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BACE > Questions on practice tests > Flashcards

Questions on practice tests Flashcards

1
Q

How do confocal microscopes work?

A

Use a laser to scan samples that have been dyed

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2
Q

How do bright field microscopes work?

A

Have objectives above stage

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3
Q

If a plasmid has 3 sites for a restriction enzyme, how many fragments will it have?

A

3

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4
Q

Electroporation

A

Used to transform bacteria

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5
Q

PCR optimal conditions

A

Priner, DNA, dNTPs, and Buffer kept constant- temp 75-95 C

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6
Q

Which of the following should NOT be used to kill DNA?
1. UV light
2. Bleach
3. Alcohol

A

Alcohol

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7
Q

Enzymes work best in a (wide/narrow) pH range

A

Narrow

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8
Q

Which will give you better seperation on DNA running bands?
1. Higher % agarose gel
2. Lower % agarose gel
3. + voltage, - running time
4. + voltage, _ running time

A

Higher % agarose gel

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9
Q

In gel electrophoresis, bigger bands move to the (top/bottom) of the gel

A

Top

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10
Q

Which can be used to deliver new DNA to a cell?
1. Lipid
2. Plasmid
3. Virus
4. All

A

All of the above

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11
Q

What is used to amplify DNA in PCR?
1. Phosphate
2. Restriction enzyme
3. Ligase
4. DNA polymerase

A

DNA polymerase

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12
Q

Can monoclonal anitbodies be produced in human cells?

A

Yes

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13
Q

Why is there a blocking step in a Western Blot?

A

To prevent non specific binding of antibodies used for detection

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14
Q

What type of polymerase is used for PCR?

A

Heat-stable DNA polymerase

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15
Q

What is a horizontal gel electrophoresis box used for?

A

Running DNA gels

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16
Q

What is a vertical gel electrophoresis box used for?

A

Protein gels

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17
Q

What does SDS page determine?

A

Size/purity of a specific protein

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18
Q

What is the time a protein stays active called?

A

Stability

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19
Q

What is the level of protein activity called?

A

Activity

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20
Q

What type of respiration uses O2 as a raw material?

A

Aerobic

21
Q

What type of respiration is a process where glucose is used by a cell?

A

Aerobic and anaerobic

22
Q

What type of respiration releases CO2 and ethyl alcohol?

A

Anaerobic respiration

23
Q

What type of respiration uses CO2 as a raw material?

A

Neither

24
Q

Prokaryotic DNA structure

A

Long, circular, 1000s o genes

25
Q

What is semi-conservative replication?

A

The process in DNA replication where each original strand serves as a template for a new strand

26
Q

When does transcription begin?

A

When RNA polymerase binds to the promoter on a DNA strand

27
Q

What enzyme is used in DNA replication?

A

DNA polymerase

28
Q

Where does translation take place in prokaryotes?

A

Ribosomes

29
Q

If a bacterial cell didn’t contain restriction enzymes, what would happen?

A

It would be easily lysed and infected by bacteriophages.

30
Q

Complementary base pairing between 2 DNA structures is due to formation of what?

A

Hydrogen bonds

31
Q

What does cellulose do?

A

Only provides energy storage and structure in cells

32
Q

Which of the following could use sugar to produce energy without O2?
1. Bacterial
2. Yeast
3. Animal (muscle)
4. All of the above

A

All of the above

33
Q

Where does protein synthesis happen?

A

The cytoplasm

34
Q

What is an epitope?

A

The part of an antigen recognized by the immune system

35
Q

A tech performs an ELISA using primary antibody with mouse Ig62a isotype to analyze patient blood samples. Which would be best?
1. Mouse anti-human
2. Mouse anti-rabbit
3. Human anti-rabbit
4. Rabbit anti-mouse

A
36
Q

(True/False)- Shaking isn’t necessary to oxygenate aerobic liquid culture for optimal growth.

A

False

37
Q

A tech is asked to determine the size of a PCR product, and runs it on a gel. The PCR band is well above (much closer to wells) than the DNA ladder bands. How should the tech proceed determining the size of a PCR product the next time samples are run?

  1. Run gel at a lower voltage
  2. Run gel at higher voltage
  3. Use a DNA ladder with larger fragments
  4. Use a DNA ladder with smaller fragments
A

3

38
Q

Is a plasmid has 2 bands, how many restriction sites does it have?

A

2

39
Q

What event caused the advent of recombinant DNA technology?

A

Isolation of restriction enzymes

40
Q

What are the steps to launch a new drug?

A

Identifying product, research and development, clinical trials, manufacturing, sales

41
Q

What are the steps to bring a biopharm product to market?

A

Research and development, process development, small scale manufacturing, large scale manufacturing

42
Q

Who regulates cGMPs?

A

FDA

43
Q

What does QA handle?

A

maintaining and controlling documentation

44
Q

What is Assay Validation?

A

Providing documented evidence that a method works

45
Q

What is the proper way to correct an incorrect date on paperwork?

A

Cross out with a single line, initial and date it

46
Q

What does a water bath do?

A

Regulate the temperature of anything subjected to heat

47
Q

Why would you only want to change part of a viral, interacting molecule on a cell?

A

To allow it to retain its original functions

48
Q

(True/false) All biohazard waste must be biologically inactivated before being disposed of as regular trash.

A

True

BACE (6 decks)

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