TCA Cycle (Catabolism of Pyruvate) Flashcards
What is the net energy and redox yield from aerobic glycolysis?
2ATP and 2NADH
What does glycolysis reduce?
NAD+ to NADH + H+
What needs to happen to NADH for glycolysis to continue?
Be oxidised to generate NAD+ = through oxidative metabolism of pyruvate
Why is NAD+ important?
Because is it an electron ACCEPTOR
Where does the TCA cycle occur?
In the mitochondrial MATRIX
How is pyruvate transported to mitochondrial matrix?
H+/pyruvate symport by faciliatated diffusion
H+ gradient from cytosol to matrix
How is pyruvate metabolised to acetyl-CoA?
By pyruvate dehydrogenase complex (PDC)
What does pyruvate dehydrogenase complex catalyse?
Oxidative decarboxylation of pyruvate to acetyle CoA
What does PDC consist of and how is it regulated?
Pyruvate dehydrogenase complex = made of 3 enzymes
Allosterically regulated by phosphorylation
What does PDC determine in well oxygenated tissues?***
Glucose oxidation
Where does PDc determine glucose oxidation?
In well oxygenated tissues
Can acetyl-CoA be converted back to pyruvate?
No, it is an irreversible reaction
What does the TCA cycle yield?
2 CO2 molecules
3 NADH molecules
1 FADH2 molecules
1 GTP
Where are enzymes of the TCA cycle located?
Mitochondrial matrix
What enzymes are located in the mitochondrial matrix?
Enzymes of TCA cycle
Except for sccinate dehydrogenase
