TCA Flashcards
1
Q
How is the binding of citrate synthase ordered?
A
oxaloacetate binds first inducing an open form for acetyl-CoA. When acetyl-CoA binds closes compactly for catalysis
2
Q
how is citrate synthase inhibited? activated?
A
inhibited:
competitively by citrate, succinyl-CoA and long chain acyl-CoA
allosterically: ATP, NADH
activated: NAP and ADP and increased substrate concentration
3
Q
how is isocitrate dehydrogenase inhibited? activated?
A
inhibited: ATP and NADH (Cooperative)
usually first to respond to NADH levels leading to citrate accumulation
activated: allosterically stimulated by ADP, NAD and Ca2+
4
Q
how is Alpha-ketoglutarate inhibited? activated?
A
inhibited: NADH, ATP, GTP, succinyl-CoA
activated: stimulated by Ca2+
