TAG

Question 1

These are 3 datty acids attached in 1 glycerol molecule

Answer 1

Triglycerides

Question 2

Measurement of TAG levels are useful when diagnosing what type of disease?

Answer 2

PRIMARY and SECONDARY hyperlipoproteinemia

Question 3

Glycerol and fatty acids are first formed by ________ action on the TAG

Answer 3

Lipase

Question 4

Glycerol is then PHOSPHORYLATED by _________________ to produce _______________ and ______________ in a reaction catalyzed by ___________

Answer 4

Adenosine-5’-triphosphate to produce glycerol-3-phosphate (G-3-P) and adenosine-5’-diphosphate in a reaction catalyzed by Glycerol Kinase

Question 5

G-3-P is oxidized by ___________ producing ________ and ____

Answer 5

Glycerylphosphate (GPO) producing Dihydroxyacetone phosphate (DAP) and H202

Question 6

Peroxide reacts with _______________ and ____________ under the influence of (catalyzer) _______ to form ____________

Answer 6

4-aminoantipyrine and 4-chlorophenol under Peroxidase (POD) to form Quinoneimine

Question 7

What are the materials needed for TAG

Answer 7

1. Triglycerides reagent
2. Triglycerides activator
3. Triglycerides Standard
4. Spectrophotometer
5. Heat block or water bath (37C)
6. Automatic pipettes
7. Cuvettes

Question 8

It is for enzyme concentrate, activator and stabilizers

Answer 8

TAG activator

Question 9

What is the TAG standard?

Answer 9

200 mg/dL

Question 10

What are the components that TAG standard contains?

Answer 10

1. Glycerol with surfactant (minimizes surface tension)
2. 0.1% sodium azide (preservative)

Question 11

At what absorbance should the spectrophotometer for TAG should be at?

Answer 11

500 nm (492-530nm)

Question 12

For TAG reagent preparation, what is the ratio of drops of TAG activator to 1 BOTTLE of TAG Reagent?

Answer 12

9:1 (9 drops ACTIVATOR is to 1 BOTTLE of REAGENT)

Question 13

For reagent preparation, what is the amount of activator that you need to put for every 5mL of reagent?

Answer 13

50UL of ACTIVATOR to every 5ML of REAGENT

Question 14

How many times should you invert reagent before use?

Answer 14

3 to 4 TIMES

Question 15

How long should you stand TAG reagent and at what temperature?

Answer 15

15 MINUTES at ROOM TEMPERATURE

Question 16

What is the volume of standard and sample to be added in cuvette?

Answer 16

both 10 uL or 0.001 ml

Question 17

What are the manual procedure for determination of TAG?

Answer 17

1. Pipette volumes into cuvettes 1 ml of activated reagent to 3 cuvettes then 10 uL of standard to standard tube and same goes with sample cuvette and mix well
2. Incubate all cuvets at 37C for 5 minutes, or if at ROOM Temp = 10 minutes
3. Read absorbance of RB, S, and U at 500 NM within 60 minutes

Question 18

What is the formula of TAG determination?

Answer 18

TAG (mg/dL) = Au/As x 200

Question 19

What is the expected values for TAG?

Answer 19

30-150 mg/dL