GLUCOSE DETERMINATION

Question 1

What are the 2 methods in glucose determination?

Answer 1

Enzymatic & Non-Enzymatic

Question 2

What method is most common test in glucose determination, but is not the reference method?

Answer 2

Glucose Oxidase Method

Question 3

What is the reference method for glucose determination?

Answer 3

Hexokinase method

Question 4

What are the substrates for primary reaction?

Answer 4

B-D-Glucose and H2O2 + O2

Question 5

What is the enzyme used for Primary reaction?

Answer 5

God - Glucose Oxidase

Question 6

What are the products of primary reaction?

Answer 6

H2O2 (Hydrogen peroxide) and Gluconic acid

Question 7

What are the color developers in secondary reaction?

Answer 7

4-aminoantipyrine + phenol

Question 8

What are the substrates of Secondary reaction?

Answer 8

H2O2 + 4-aminoantipyrine + phenol

Question 9

What is the enzyme for the secondary reaction?

Answer 9

POD - Peroxidase

Question 10

What are the end products of the secondary reaction?

Answer 10

Quinonime complex + H2O

Question 11

What quinone complex color formed at secondary reaction?

Answer 11

Red-violet

Question 12

What is the relationship of the intensity of color to the glucose concentration?

Answer 12

Proportional

Question 13

What is the purpose of phosphate buffer in the experiment?

Answer 13

To neutralize the pH level

Question 14

What is the glucose standard, and wavelength?

Answer 14

100mg/dL ; 500 nm

Question 15

The wavelength 500 NM is under what spectrum?

Answer 15

Visible spectrum

Question 16

Enumerate the manual procedure of glucose oxidase method.

Answer 16

1. Zero spectrophotometer at 500 NM with distilled water.
2. For each standard and sample tubes, add 1.0 mL reagent to test tubes.
3. Warm the tubes to 37C for 5 minutes
4. Add 10 uL (0.010 mL) of SERUM in SAMPLE TUBE, while 10 uL (0.010 mL) of GLUCOSE STANDARD in STANDARD TUBE.
5. Return to 37C incubation for 5 minutes.
6. After 5 minutes of incubation, read and record the absorbance of all samples.

Question 17

T or F: 37C = 99F = 310.15K

Answer 17

TRUE

Question 18

What is the molecular formula of phenol?

Answer 18

C6H5OH

Question 19

What is the molecular formula of 4-aminoantipyrine

Answer 19

C11H13N3O

Question 20

What is the principle of result for glucose oxidase method?

Answer 20

To compare the Au with As that is identically treated.

Question 21

What is the formula of glucose determination? With unit.

Answer 21

Glucose (mg/dL) = Au/As x 100

Question 22

What is the reference range of serum/plasma, and its’ equivalence to mmol/L?

Answer 22

70-105 mg/dL ; 3.89 - 5.83 mmol/L

Question 23

What is the reference range of CSF, and its’ equivalence to mmol/L?

Answer 23

40-75 mg/dL ; 2.22-4.17 mmol/L

Question 24

Result: 70-105 mg/dL is read as?

Answer 24

Normal

Question 25

Result: <70 mg/dL is read as?

Answer 25

Below normal

Question 26

Result: >105 mg/dL is read as?

Answer 26

Above normal