HDL

Question 1

What is the major lipoprotein of HDL that is also known as transporter of lipids?

Answer 1

Alpha-lipoprotein

Question 2

HDL is considered as a good or bad cholesterol?

Answer 2

GOOD

Question 3

What is the required fasting hour for HDL determination?

Answer 3

12 hours

Question 4

What is the purpose of HDL determination?

Answer 4

To remove excess tissue

Question 5

Enumerate the separation method for HDL Determination

Answer 5

1. In one test tube, add: 500uL (0.5ml) of sample (SERUM), and 50uL (0.05ml) of HDL precipitating agent.
2. Vortex to properly mix
3. Stand for 5 minutes at ROOM TEMPERATURE (22C)
4. Centrifuge for 10 minutes using 3400 RPM
5. Aspirate the SUPERNATANT

Question 6

What is the purpose of of HDL precipitating agent in HDL separation method?

Answer 6

For specificity of HDL and avoid interference.

Question 7

What is the purpose of Vortex for the HDL separation method?

Answer 7

To properly mix the reagent

Question 8

Why is it important to avoid disturbing the sediment while aspirating the supernatant?

Answer 8

if disturbed, result will FALSELY INCREASE

Question 9

In adding supernatant and HDL standard to tubes for standard and sample, what is the amount of volume that you need to add?

Answer 9

25uL = 0.025 mL

Question 10

What is the HDL standard? with unit

Answer 10

50mg/dL

Question 11

At what wavelength of HDL can be read at?

Answer 11

500 nm

Question 12

What is the formula for the HDL determination?

Answer 12

AHDL / AS x 55 (50 if not converted with dilution factor)

Question 13

What is the dilution factor for HDL?

Answer 13

1.1