T cell differentiation and function

Question 1

STATs for TH1 differentiation

Answer 1

STAT1 and STAT4

Question 2

Cytokines for TH1 differentiation

Answer 2

IFNg (Stat1) and IL-12 (Stat4)

Question 3

Induction of TH1 program

Answer 3

STAT1 and Stat4 can both induce Tbet
Tbet (hlx, Runx3, Ets) drive expression of IFNg and repress IL4
-Tbet represses function of Gata and RORc
-ITK - pTbet and which supresses gata3

Question 4

STATs for TH2 differentiation

Answer 4

STAT5 or STAT6 can induce gata3

Stat3 also contributes to TH2 differentiation

Question 5

Alternative induction of gata3

Answer 5

Notch can induce gata3 independent of stat6

Question 6

Cmaf

Answer 6

Cmaf is a TF
expression driven by gata3
-drives expression of IL4
-not required for other TH2 cytokines

Question 7

Cytokines for TH17

Answer 7

IL6 and IL23

Question 8

STAT for TH17

Answer 8

STAT3 (induced by both IL6 and IL23)

Question 9

Transcription factors for IL17 expression

Answer 9

Aryl Hydrocarbon receptor, Batf, IkBz, IRF4, Runx1, all promote IL17 expression

Question 10

Inhibitors of TH17 program

Answer 10

Foxp3, GF1, Ets1

TCF-1 directly binds IL17 gene, also represses IL7r, thus constraining the survival

Question 11

Induction of the treg program

Answer 11

IL-2 signals through stat5

Question 12

Foxp3 maintenance of Treg program

Answer 12

• interferes with rorc (and TH17 development)

- directly regulates genes important for the treg program

Question 13

Runx1 and Treg program

Answer 13

Runx1 can either interact with rorc to promote Th17 or with Foxp3 to promote Treg
-Runx proteins can also regulate expression of rorc and foxp3

Question 14

Foxo and Treg program

Answer 14

Foxo1 and foxo3 promote tregs

Question 15

Tregs and lineage supression

Answer 15

Tregs supress other effector responses using components of the transcriptional machinery that are important for the target effector response

Question 16

Sat1b

Answer 16

Chromatin organizer that is repressed by foxp4 so that it can’t remodel chromatin at cytokines that drive other effector lineages

Question 17

Surface phenotype associated with enhanced CD8 memory generation

Answer 17

CD127hi CD27+ KLRG1lo

Also express BCL2

Question 18

Requirements for CD8 memory maintenance

Answer 18

Antigen independent

IL-7 and IL-15 dependent

Question 19

Tcm

Answer 19

Central memory T cells - greater proliferative potential upon reactivation but lower expression of effector cytokines. Express high levels of IL-2 and have high migratory capacity

Question 20

Tem

Answer 20

effector memory T cells that constitutively express cytotoxic proteins. Decreased proliferative potential and IL-2 production. High migratory capacity and effector function/cytotoxicity
these will be enriched upon repetitive vaccinations or successive infections.

Question 21

Tcm Surface phenotype

Answer 21

CD62Lhi CCR7 hi

Question 22

Tem surface phenotype

Answer 22

CD62L low CCR7 low

Question 23

Trm

Answer 23

Tissue resident memory cells that do not recirculate
Decreased proliferation, IL-2 production and migration
increased effector functions and cytotoxicity

Question 24

Trm surface phenotype

Answer 24

CD103hi CD69hi CD62Llow CD27low

Tissue specific homing receptors

Question 25

Tcm location

Answer 25

LN, spleen blood

Question 26

Tem location

Answer 26

Spleen, blood, liver

Question 27

Trm

Answer 27

Skin, gut, brain

Question 28

main proinflammitory cytokines during viral/other intracellular infections

Answer 28

Type I IFN, IFNg, IL-2, IL-12, IL-27, IL-33

Question 29

TH1 SOCS member

Answer 29

SOCS5

Question 30

TH3 SOCS member

Answer 30

SOCS3

Question 31

TH17 SOCS member

Answer 31

SOCS1