Surface electric charge of cells. Electrophoresis and its applications in medicine Flashcards
Origin of the electric charge on the cell surface
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Electric charge of proteins
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Proteins in acidic solutions
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Proteins in basic solutions
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Electric charge of lipids and hydrocarbons
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Electric charge of lipids and hydrocarbons

Potential determining ions

Counterions

Electric charge on the cell surface

Electrical double layer

Structure of the electrical double layer

The stern layer and the diffuse layer

The Gouy-Chapman model for the double layer

Distribution of the counterions

Equilibrium of counterions

Equilibrium distribution of the electrical potential

The debye screening length

Equilibrium distribution of the counterions

Density of the sruface charge

Electrical potential in the cell and insdide the membrane

Cell motion in an electric field

The electrokinetic potential

Measurement of the electrokinetic potential

Electrophoresis

Electrophoretic mobility

Forces acting on the moving particle

The electric force

The drag force

The electrophoretic mobility

Measurement of the electrophoretic mobility

Isoelectric point

Stability of cell suspensions

Applications of electrophoresis in medicine

Molecular electrophoresis

Paper electrophoresis

Immunoelectrophoresis
