Super resolution microscopy and neuroplasticity research Flashcards
What is an Abbe’s limit of diffraction?
A fundamental limit of an optical imaging system. Even if you keep magnifying things, they’ll continue to look larger, but you won’t necessarily see more detail
Whats is resolution?
The shortest distance between two points that can be distinguished. The shorter this distance, the better the resolution.
the smallest interval measurable by a telescope or other scientific instrument; the resolving power
Which technique allows us to see smaller details than as visible with light microscopy?
Electron microscopy.Electrons have much smaller waves than light and allow a much higher resolution. If you remember, the light wave length has a bearing on the resolution that we can achieve. So, this method works by effectively replacing the light with a beam of electrons and the samples may be labelled with metals, as many biological samples are transparent otherwise
Limitations of electron microscopy.
samples have to be fixed (dead)
you can’t label multiple different structures easily
What is super resolution?
Techniques that can allow us to see structures beyond the limit of resolution for normal light microscopy. For example - Locallisation microscopy.
How using the point spread function works?
What are the two commonly referred techniques in locallisation microscopy?
What is plasticity?
The ability of the nervous system to adapt to sensory experience.
What are AMPA receptors?
They’re glutamate receptors in excitatory synapses. They’re voltagegated ion channels, which are enriched in the postsynaptic membrane, and they’re trafficked in and out of synapses in an activity dependent manner.
