Success In Biology Flashcards
Endosymbiotic Theory
Organelles of eukaryotes evolved through symbiosis of prokaryotes.
Similarities between mitochondria, chloroplast and prokaryotic cells.
Plasma membranes are amphipathic
Posses both hydrophilic (water-dissolving, polar) and hydrophobic (water-resistant, non-polar) regions.
Peripheral Proteins
- more of these than integral proteins.
- surface proteins.
- glycoproteins.
Integral Proteins
- Completely embedded
- Transmembrane
a. transport proteins (i.e protein channels)
b. some glycoproteins
c. membrane receptors - When cross lipid bilateral takes alpha helix shape.
Glycoproteins + Glycolipids
- Found on extra-cellular surface of membranes.
- Proteins and lipids have short carbohydrate chains extending out.
- Form OH bonds with water surrounding cells, reinforcing cell structure.
Membrane receptors (integral proteins)
- Bind to molecules outside of cell and transfer messages from outside to inside cell.
a. Ligand-gated ion channel receptors
b. Enzyme-coupled receptors
c. G-protein coupled receptors.
Lipid-Raft Theory
Lipid rafts are dense regions of the plasma membrane that are heavy in cholesterols and serve as protein signaling platforms.
Osmosis and Aquaporins
Osmosis is movement of water molecules across a selectively permeable membrane into a compartment containing solute unable to cross that membrane
a. Aquaporins: transport proteins allowing water in and out ( not gated; open/close).
Osmolarity vs Osmolality vs Tonicity (Terms describing solute concentrations)
Osmolarity: solute concentration per volume of solvent.
Osmolality: solute concentration per mass of solvent.
Tonicity: Ability of solution surrounding a cell to cause the cell to gain or lose water.
Isotonic (osmosis)
Concentration of solute is same inside and outside of cell
Hypertonic (osmosis)
Concentration of solute is higher outside the cell than inside (cell shrivels)
Hypotonic (osmosis)
Concentration of solute is higher inside the cell than outside (cell bloats)
Diffusion
Movement of molecules or ions in liquid or gas from areas of high concentration to lower concentration.
a. Occurs down a concentration gradient.
b. Cell membrane permeable to only that substance.
C. O2 diffuses in and CO2 out of cell.
Facilitated Diffusion
Substances unable to pass through lipid bilayer need help from membrane proteins.
a. ion channels
b. Carrier molecules: for molecules not lipid-soluble and too big e.g glucose
c. Insulin promotes facilitated diffusion of glucose.
d. Specific because carrier molecule only binds to certain solute.
Filtration
Forces molecules through membranes by exerting pressure (hydrostatic).
a. Blood pressure generated by heart action
Active Transport
Moves from regions of lower concentrations to higher concentrations through membrane.
a. Uses carrier molecules and ATP
b. Transported are: sugars, amino acids, and ions.
Endocytosis + Exocytosis
Processes using cellular energy to move substances in/out of cell without crossing cell membrane.
a. Endocytosis: particles too big to enter via diffusion or active transport are conveyed in a vesicle formed from a portion of the membrane. (Types: pinocytosis, phagocytosis, receptor-mediated endocytosis).
b. Exocytosis: secretes substance stored in vesicle from cell. e.g nerve cells secreting neurotransmitters.
Pinocytosis (Endocytosis)
- “Cell drinking.” Cells take in tiny droplets of liquids from surroundings.
- Takes in water and substances dissolved in it too large to enter.
- Vesicle forms from portion of cell membrane
Phagocytosis (Endocytosis)
- “Cell eating.” Cell takes in solids.
2. Portion of cell membrane projects outward once solid particle comes into contact and draws inside.
Receptor-mediated Endocytosis (Endocytosis)
- Moves very specific kinds of particles into cell.
- Protein molecules extending to outer surface of cell membrane act as receptor that specific molecules bind to (ligand)
- Cholesterol enters this way.
Passive Transport
Molecules move in and out of cell according to concentration gradient.
a. Moves by Kinetic energy and NO ATP REQUIRED.