Study Guide

Question 1

Limiting factors to cytology sampling

Answer 1

Not always representative of lesions

May not be diagnostic (mixed cell/low cell/ hemodiluted)

Suseptable to poor handling and preparation (thick)

Question 2

Sample tube for culture

Answer 2

Plain red top

Question 3

What tube for cytology (when cell count wanted)

Answer 3

Purple top (EDTA)

Question 4

Why make slides at time of collection for fluid samples

Answer 4

Limit cellular swelling and artifacts

Question 5

What is smear artifact ? Cause? What sample most commonly effected?

Answer 5

Nuclear streaming from ruptured cells via excessive tissue handling

Occurs more often in LN aspirated (esp lymphoma)

Question 6

When is heat fiction neccesary?

Answer 6

Waxy samples (ie ear)

Question 7

Steps for prepping tissue imprint for cytology from biopsy sample

Answer 7

Blot excess fluid on clean gauze before imprinting the internal cut surface in linear fashion before exposing to formalin

Question 8

Why is clinical history important

Answer 8

Help differentiate cytologically similar samples

Question 9

What is Koehler illumination

Answer 9

Focuses light for best picture quality

Question 10

FNA w/ suction vs w/o

Answer 10

W/O syringe allow better control, less trauma and aspiration in differnt tissue layers