Streak Inoculation

Question 1

What is used to grow a bacteria culture?

Answer 1

Agar

Question 2

What is the most commonly used agar type?

Answer 2

Nutrient Agar

Question 3

What should you label an agar plate with?

Answer 3

The date, your initials, the name of the culture (e.g. sample A)

Question 4

How do you ‘streak out’ a bacteria colony?

4

Answer 4

Flame a wire loop

Using the loop pick up a single colony and then streak it on to the fresh agar plate by using a back and forward movement

First draw two lines then streak out for a couple lines

Turn the agar plate and repeat the process making sure you overlap each side

Question 5

What should you do after streaking out your colony?

Answer 5

You need to incubate your plate at whatever temperature is required (usually at 37 degrees)

Question 6

Why is streak-inoculation done ?

Answer 6

It is done to achieve a pure culture which can be subjected to basic characterisation and further tests