Strand 1

Question 1

Function of microscope?

Answer 1

Enables us to see the specimen that are difficult to be see with naked eye

Question 2

What are the 2 functions of microscope?

Answer 2

Magnification and resolution

Question 3

Differentiate between magnification and resolution?

Answer 3

Magnification - enlarges the object. ( my note: it only zooms)

Resolution - ability to differentiate between 2 things that appears as one with naked eye ( my note: it makes things appear clear)

Question 4

List the 4 steps of preparing wet mount?

Answer 4

1. Clean the cover slip and dry it , than add a drop of water or stain
2. Place the specimen in the drop water or
   Stain
3. Lower the coverslip at an angle of 45• to avoid air bubbles being trapped in
4. Observe wet mount under microscope

Question 5

What are the steps of focussing wet mount?

Question 6

Can wet mount be viewed at 100x?

Answer 6

No , but the oil immersion can be viewed at 100x which improves its image clarity and detail

Question 7

Why should you lower wet mount at an angle of 45•?

Answer 7

To avoid air bubbles being trapped in so that you can get a clean / clear view of specimen

Question 8

Function of coarse adjustment knob?

Answer 8

Makes large changes to bring specimen into focus

Question 9

Function of fine adjustment knob?

Answer 9

Makes small changes to sharpen the image

Question 10

Function of nose piece?

Answer 10

Holds and rotates the objective lens for different magnification

Question 11

Function of eyepiece?

Answer 11

Is the lens you look through when viewing specimen

Question 12

Difference between high power field of view and low power field of view?

Answer 12

High power field of view - produces larger FOV meaning you can see broader area of specimen

Low power Fov - produces a smaller FOV , more detailed region of the specimen

Question 13

Staining used for animal and plant?

Answer 13

Animal - methylene blue

Plant -iodine solution