Stertz Flashcards
How many major proteins encoded by IAV ?
11.
Entry mode for IAV ?
Receptor mediated endocytosis.
Reminder, who does the cap snatching?
PB2 binds the caps and PA cuts them.
How is splicing done for IAV?
Basically to make the few accessory proteins they just use the cellular machinery. It’s not super efficient with viral transcripts tho, so you get both variants of the protein produced.
Stronger binding HA to sialic acids require, as a consequence…
… A more active NA.
What’s the evolutionary plasticity of HA compared to MeV glycoproteins ?
It’s reaaally super loose. It can handle a shit ton of mutagenesis without any problems, whereas Measles just hates it.
What’s a subtype of IAV referred to?
Type of HA and NA, and there’s no cross reactivity of antibodies between subtypes.
What’s the fun little thing about H7N9 receptor binding ?
It’s an avian strain that binds quite well to human sialic acids.. Pandemic much ?
what’s the error rate of IAV RdRp ?
10 000 nucleotides 1 error.
what are the two approaches to generate a potentially universal IAV vaccine ?
Chimeric HA approach or headless HA approach.
Name a neuraminidase inhibitor and a PA inhibitor
Oseltamavir (NA)
Baloxavir (PA)
Are HAV, HBV and HCV all part of the same happy family ?
No - A is a picorna, B a Hepadna and C a Flavivirus. Even their genomes are different, as HBV has a dsDNA genome.
So HBV is a bit shit. Only 5% of infected people don’t clear the virus and get symptoms… However it has another, more grave effect, which is it ?
HBV infection increases the risk of Hepatocellular carcinoma at all stages of infection - might be due to its DNA integration or of persistant liver damage sustained during chronic infections.
Give a structure overview of HBV virions.
Small (40 nm) enveloped virus, with three glycoproteins: LMS (Large Middle Small).
3kb genome. partially dsDNA.
What is CCC DNA ?
Covalently Closed Circular DNA. it’s the template for transcription by the polII.