Fraefel

Question 1

HSV1 entry ?

Answer 1

Fusion after using HveA/C receptors.W

Question 2

HSV1 DNA replication: how ?

Answer 2

Circularisation of the genome and binding of Origin binding protein and ssDNA briding protein. The DNA Helicase and Polymerases get then recruited and a rolling circle replication begins.

Question 3

What’s HSV1 neuroinvasiveness and virulence like ?

Answer 3

Quite low invasiveness but high virulence.
Can effectively enter sensory nerves, uses retrograde transport then cause a life long latent infection. Can become chronic if it wakes up and uses anteretrograde transport.

Question 4

What’s going on with HSV1 gene expression during latency ?

Answer 4

all viral transcripts are silenced excepted for the LAT transcript. This one encodes a few RNA species, including miRNA, to silence lytic genes. Stresses might reactivate the virus, and degrade LAT transcripts.

Question 5

genome size ?

Answer 5

dsDNA of 152 kbp with 87 genes !!

Question 6

Which protein is responsible for fusion ?

Answer 6

Gb.

Question 7

what are the three classes of herpesviruses and their targets=

Answer 7

Alpha : HSV1 for example. epithelial and sensory neurons infections. latent.

beta: HHV6 for example. kidneys, secretory organs.
gamma: B cells: EBV and HHV8. oncogenic.

Question 8

What’s the tegument protein?

Answer 8

It’s the VP16 and is packaged in virions. Its promoter gets reactivated during latency if stress. doesn’t happen in neurons tho, and it stay in heterochromatin.
It’s a transactivtor, binds IE promoters.

Question 9

What’s ICPO?

Answer 9

That’s HSV1 IE protein, responsible for transcription activation. Mediates also host factors by targeting them for proteosome degradation.

Question 10

what are the two uses of HSV1 in gene therapy?

Answer 10

usage as recombinant vectors, single cycle to target cells.

or usage as amplicons, no viral genes and requires a helper virus.