steps of PCR TESTING

Question 1

step 1- denaturation

Answer 1

denaturation, 1 minute

dna is heated to 94 degrees to break bonds and seperate strands

Question 2

step 2 - annealing

Answer 2

annealing, 2 minutes
single strands are cooled allowing primers to join together

Question 3

step 3 - elongation

Answer 3

1 minute - dna is heated again, taq polymerase binds to primer and makes new complementayr strand

Question 4

finally

Answer 4

ends up in copy of stage 1

Question 5

Gel electrophosis

Answer 5

its a tub, with agar gel and markers, base pairs need to add up to check i pcr was done good