CRISPR

Question 1

what is CRISPR

Answer 1

clustered regularly interspaced short palondromic repeats

Question 2

what does it do

Answer 2

plays a role in immune response of bacteria against viruses

Question 3

what are the steps and components of crispr cas 9

Answer 3

CAS 1 AND CAS 2 cut out segments of virus dna , which are then put into the spacers of the palondromic sequence

Cas1 and Cas2 identify a specific sequence called the PAM (e.g., “GG”) to cut the viral DNA accurately.
The PAM is crucial for distinguishing between foreign viral DNA and the bacterium’s own DNA.

cas 9 then travels to virus dna sequence and cas 9 cuts the virus, CAS 9 IS ITS OWN STRUCTURE

Question 4

artifical crispr

Answer 4

A guide RNA (sgRNA) is designed to match a specific 20-nucleotide sequence in the DNA you want to edit. This sequence is called the protospacer.
Attach Guide RNA to Cas9

The sgRNA binds to the Cas9 enzyme, that can locate and cut the target DNA.
Find the Target DNA Sequence

The Cas9-sgRNA searches the DNA for a matching sequence. For Cas9 to cut, the target DNA must be near a PAM
Cas9 Cuts the DNA

Cas9 makes a double-stranded break at the target site once it finds the correct match with the guide RNA and PAM sequence.
Cell Repairs the DNA