Steps in Viral Replication

Question 1

Steps in Viral Replication

Answer 1

1. Attachment
2. Penetration
3. Uncoating
4. Macromolecular synthesis
5. Viral assembly
6. Lysis & Release

Question 2

• recognition of host cell and specific binding between viral capsid proteins and the receptor of the host cell.

Answer 2

Attachment

Question 3

Viruses enter the host cell

Answer 3

Penetration

Question 4

process by which the capsid is removed

Answer 4

Uncoating

Question 5

Note:

Answer 5

• RNA viruses usually release the genome into the cytoplasm, whereas most
• DNA viruses release their genome into the host nucleus

Question 6

• production of nucleic acid and protein polymers.

Answer 6

Macromolecular synthesis

Question 7

• process by which structural proteins, genomes, and in some cases viral enzymes are assembled into virus particles

Answer 7

Viral assembly

Question 8

• new virions are then released by lysis if they are naked viruses or by budding iif they are enveloped viruses.

Answer 8

Lysis & Release

Question 9

Specimen Collection and Transport of viruses

Answer 9

o Early stage of infection before height of fever
o Use dacron/rayon swab (do not use calcium alginate swabs)
o Storage: 4°C for 5 days;
- if >6 days = 20°C or –70°C
o Transport: -70°C

Question 10

Transport medium for viruses

Answer 10

LEASHU

o Leibovitz-Emory
o Eagle’s Tissue Culture
o Amie’s
o Stuart
o Hank’s (buffered isotonic solution with albumin, gelatin)
o Universal Transport Media (UTM)