Laboratory Diagnosis for Viruses Flashcards

1
Q

Most rapid method of diagnosis for non- culturable viruses (hepatitis virus, norwalk virus)

A

Electron Microscope

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2
Q

Detection of cytoplasmic inclusions/cytopathic effects

A

Light Microscopy

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3
Q

Negri Bodies

A

rabies

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4
Q

Torres Councilman Bodies

A

Yellow fever

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5
Q

Cowdry Type A

A
  • HSV Herpes Simplex Virus
  • VZV Varicella Zoster Virus
    (Tzanck smear)
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6
Q

Owl’s eyes

A

CMV

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7
Q

Koilocytes

A

HPV (Pap smear)

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8
Q

Henderson-Patterson Bodies

A

Molluscum contagiosum (poxyviridae)

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9
Q

Guarnieri bodies

A

Pox Virus

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10
Q

For detection of HPV carcinoma

A

Pap Smear

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11
Q

For HSV & VZV; multinucleated giant cell

A

Tzanck smear

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12
Q
  • obtained from animals
  • Primary Monkey Kidney
  • Human Embryonic Kidney
  • Rhesus Kidney
A

Primary Cell Culture

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13
Q
  • subcultivated primary culture
  • MRC-5, WI-38,
  • Human Diploid Fibroblast (HDF)
A

Diploid Cell Culture

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14
Q
  • immortal cell lines
  • cancer cells
  • HeLa, Hep2, A-549, AGMK
A

Continuous Cell Culture

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15
Q

detects blockade of cytopathic effects Complement fixation

A

Neutralization

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16
Q

for influenza and rubella

A

Hemagglutination inhibition

17
Q
  • Presence of viral DNA or RNA is the gold standard in viral diagnosis
A

Nucleic acid detection

18
Q

DNA Viruses

A

refer to handout page 5

19
Q

RNA Viruses

A

refer to handout page 5-6