STEP 8: MOUNTING Flashcards by Antea Eper

Q

Is used to adhere fecal specimens to glass slides in the Modified Iron Hematoxylin

A

Mayer’s Egg Albumin

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Q

Most common, easy and convenient, relatively inexpensive adhesive

A

Mayer’s Egg Albumin

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Q

prevents the growth of mold in Mayer’s egg albumin

A

Thymol

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Q

Recommended for use with paper artifacts

A

Starch Paste

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Q

Utilized for Immunohistochemistry
It has also been reported to improve protein coating of ELISA plates

A

Poly-L-lysine

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Q

Adhesive used for cytological studies for proteinaceous or bloody material

A

APES (3-aminopropyltriethoxysilane)

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Q

Most commonly used adhesive

A

Albumin

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Q

Disadvantage of albumin

A

Retains some stain and give dirty background

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Q

APES concentration used

A

Slides are dipped in 2% APES

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Q

The most convenient alternative to direct coating of slides with gelatin

A

Adding 30 mL of 1% aq gelatin to water in the floating bath

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Q

Corrosive adhesive

A

APES (3-aminopropyltriethoxysilane)

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Q

Refractive index for mounting media that enhances specimen details or make them invisible

A

1.518

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Q

Mounting media must always have a refractive index ______ than the mounted sample to impart more transparency

A

Higher

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Q

Characteristics of a good mounting media:

It should be freely miscible with ________ and ________

A

Xylene and toluene

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Q

Characteristics of a good mounting media:

It should not dry to a __________

A

Non-stick consistency and harden relatively quickly

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Q

Characteristics of a good mounting media:

It should be resistant to _________

A

Contamination

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Q

Characteristics of a good mounting media:

It should not change in _________ and __________

A

Color and pH

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Q

Characteristics of a good mounting media:

It should be compatible with the ___________ in use

A

Adhesive

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Q

10 Characteristics of a good mounting media

A

It should be colorless and transparent
It should be freely miscible with xylene and toluene
It should not dry to a non-stick consistency and harden relatively quickly
It should protect the section from physical damage and chemical activity
It should be resistant to contamination
It should not cause shrinkage and distortion of tissues
It should not leach our any stain or affect staining
It should not change in color or pH
It should be compatible with the adhesive in use
It should set without crystallizing, cracking or shrinking, and not react with, leach or induce fading in stains and reaction products

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Q

Used for mounting sections from distilled water when stains would be decolorized or removed by alcohol and xylene

A

Aqueous mounting media

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Q

Composition of aqueous mounting media

A

Gelatin
Glycerin jelly or gum arabic
Glycerol
Sugar
Preservative solution

Q

Purpose of sugar in Aqueous mounting media

A

Increase the refractive index

Q

Purpose of glycerin jelly or gum arabic in Aqueous mounting media

A

To solidify the medium

Q

Purpose of glycerol in Aqueous mounting media

A

To prevent cracking and drying of the preparation

Glycerin refractive index

1.46

Very suitable semi-permanent mounting medium; usually regarded as the standard mountant for fat stains

Glycerin

Commonly used to mount sections for immunofluorescence

Phosphate buffer glycerol

Phosphate buffer glycerol refractive index

1.47

Standard mounting medium used when dehydration and clearing with xylene cannot be made (as in fat stains)

Glycerin jelly

Polyvinyl alcohol refractive index

1.5

Alternative for glycerin jelly, commonly used for fluorescent labels with paraphenylenediamine as an antifading agent

Polyvinyl alcohol

Farrant’s medium (gum arabic) refractive index

1.34

Does not solidify upon storage and therefore does not need to be heated before use

Farrant’s medium

In Farrant’s medium, addition of ____________ (50 mg) will produce a neutral pH instead of an acid medium raising the refractive index to 1.44

Potassium acetate

Apathy’s medium refractive index

1.52

Used for methylene blue-stained nerve preparations

Apathy’s medium

One of the most useful aqueous mountants for fluorescent microscopy, being virtually non-fluorescent

Apathy’s medium

Recommended for mounting frozen sections from water

Brun’s fluid

Frozen sections mounted directly from water or paraffin sections should be mounted on…

Glycerin, gum syrup or Brun’s fluid

Used for preparations that have been dehydrated and cleared in xylene or toluene, and are recommended for majority of staining methods

Resinous mounting media

Resinous mounting media refractive index

1.518

What type of resin is used for embedding undecalcified bones, and for electron microscopy

Synthetic resin

Canada balsam refractive index

1.524

Mounting media recommended for whole mounts and for thick sections

Canada balsam

Dibutyl, Phthalate, and Xylene (DPX) refractive index

1.532

Recommended for small tissue sections but not for whole mounts Should be used in excess amounts Colorless, neutral medium in which most standard stains are well preserved

Dibutyl, Phthalate, and Xylene (DPX)

XAM refractive index

1.52

Synthetic resin mixture in xylene (pale yellow or colorless solution) It dries quickly without retraction, and preserves stains well

XAM

Clarite refractive index

1.544

Synthetic resin which is soluble in xylene (it is used as a 60% solution in xylene), and is generally preferred over DPX

Clarite