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HTMLE > STEP 6: SECTIONING (source: ruthie) > Flashcards

STEP 6: SECTIONING (source: ruthie) Flashcards

1
Q

simplest microtome

A

rocking

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2
Q

for cutting large blocks of paraffin embedded tissues (for gelatin and celloidin)

A

rocking

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3
Q

invented by Paldwell Trefall

A

rocking

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4
Q

invented by Minot

A

rotary

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5
Q

most common microtome; used for routine and research

A

rotary

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6
Q

knife used for rotary microtome

A

biconcave knife

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7
Q

cut paraffin embedded tissues

A

rotary

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8
Q

invented by Adams

A

sliding

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9
Q

most dangerous type of microtome

A

sliding

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10
Q

uses biconcave knife; for cutting celloidin embedded tissue

A

sliding

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11
Q

recommended for cutting extremely hard and rough tissue blocks

A

sliding

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12
Q

movable block; stationary knife

A

base-sledge sliding microtome

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13
Q

stationary block; movable knife

A

standard sliding microtome

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14
Q

invented by Queckett

A

freezing

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15
Q

used for undehydrated and unembedded frozen tissues

A

freezing

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16
Q

what knife is used for freezing microtome?

A

plane-wedge knife

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17
Q

allows for easier and faster sectioning

A

cryostat

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18
Q

temperature maintaned for cryostat

A

-5 to -30C (average -20C)

capable of freezing tissues within 2-3 minutes

19
Q

optimum workin temperature for cryostat

A

-18 to -24C

20
Q

most commonly used for rapid preparation of urgent tissue biopsies for intraoperative diagnosis

A

cryostat

21
Q

equipped with a glass or gem grade diamond knife is used to cut very thinn sections of tissue mebedded in epoxy resin

A

ultrathin microtome

22
Q

for cutting plastic embedded tissues for electron microscopy

A

ultrathin microtome

23
Q

microtome knife used for cutting celloidin-embedded tissue blocks on sliding microtome

A

plane-concave

24
Q

microtome knife used to cut paraffin sections on base-sledge, rotary or rocking microtome

A

plane-concave

25
Q

microtome knife used for frozen sections

A

plane-wedge

26
Q

microtome knife used for cutting extremely hard and tough specimens embedded in paraffin blocks

A

plane-wedge

27
Q

microtome knife used for cutting paraffin-embedded sections on a rotary microtome

A

biconcave

28
Q

microtome knife used for trimming and semi-thin sectioning of tissue blocks for electron microscopy

A

glass knives

29
Q

microtome knife used to cut any type of resin block for electron microscopy

A

diamond knives

30
Q

angle formed between the cutting edges

A

bevel angle

31
Q

bevel angle

A

27-32º

32
Q

theoretically, the perfect and optimum cutting angle

A

wedge angle

33
Q

angle with maximum penetration of tissues and minimizing distortion

A

wedge angle

34
Q

wedge angle

A

15º

35
Q

inclination of cutting plane to avoid compression of the block

A

clearance angle

36
Q

prevent uneven sections, or alternate thin and thick sections

A

clearance angle

37
Q

clearance angle

A

5-10º

38
Q

For the removal of gross
Smooth stones, Machine hone
Soapy water, Oil (Mineral oil, Castor oil, Clove oil), Xylene, Liquid Paraffin
Heel-to-Toe
Zigzag

A

honing (hard sharpening)

39
Q

For the removal of irregularities/blurr
Shell horse leather
Vegetable Oil
Toe-to-Heel
Zigzag

A

stropping

40
Q

water bath temperature

A

45-50ºC
temperature should be between 5-10ºC below the melting point of the paraffin wax (56-65ºC)

41
Q

done to flatten the sections and prepare them for mounting

A

floating out/fishing out (water bath)

42
Q

done to fix the tissues into the slides. may use incubator, paraffin oven, hot plate.

A

drying

43
Q

to remove paraffin and render the tissue penetrable by the stain. done by immersing the slides into xylol (descending grades of alcohol)

A

deparaffinization

HTMLE (12 decks)

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