STEP 6: SECTIONING (source: ruthie)

Question 1

simplest microtome

Answer 1

rocking

Question 2

for cutting large blocks of paraffin embedded tissues (for gelatin and celloidin)

Answer 2

rocking

Question 3

invented by Paldwell Trefall

Answer 3

rocking

Question 4

invented by Minot

Answer 4

rotary

Question 5

most common microtome; used for routine and research

Answer 5

rotary

Question 6

knife used for rotary microtome

Answer 6

biconcave knife

Question 7

cut paraffin embedded tissues

Answer 7

rotary

Question 8

invented by Adams

Answer 8

sliding

Question 9

most dangerous type of microtome

Answer 9

sliding

Question 10

uses biconcave knife; for cutting celloidin embedded tissue

Answer 10

sliding

Question 11

recommended for cutting extremely hard and rough tissue blocks

Answer 11

sliding

Question 12

movable block; stationary knife

Answer 12

base-sledge sliding microtome

Question 13

stationary block; movable knife

Answer 13

standard sliding microtome

Question 14

invented by Queckett

Answer 14

freezing

Question 15

used for undehydrated and unembedded frozen tissues

Answer 15

freezing

Question 16

what knife is used for freezing microtome?

Answer 16

plane-wedge knife

Question 17

allows for easier and faster sectioning

Answer 17

cryostat

Question 18

temperature maintaned for cryostat

Answer 18

-5 to -30C (average -20C)

capable of freezing tissues within 2-3 minutes

Question 19

optimum workin temperature for cryostat

Answer 19

-18 to -24C

Question 20

most commonly used for rapid preparation of urgent tissue biopsies for intraoperative diagnosis

Answer 20

cryostat

Question 21

equipped with a glass or gem grade diamond knife is used to cut very thinn sections of tissue mebedded in epoxy resin

Answer 21

ultrathin microtome

Question 22

for cutting plastic embedded tissues for electron microscopy

Answer 22

ultrathin microtome

Question 23

microtome knife used for cutting celloidin-embedded tissue blocks on sliding microtome

Answer 23

plane-concave

Question 24

microtome knife used to cut paraffin sections on base-sledge, rotary or rocking microtome

Answer 24

plane-concave

Question 25

microtome knife used for frozen sections

Answer 25

plane-wedge

Question 26

microtome knife used for cutting extremely hard and tough specimens embedded in paraffin blocks

Answer 26

plane-wedge

Question 27

microtome knife used for cutting paraffin-embedded sections on a rotary microtome

Answer 27

biconcave

Question 28

microtome knife used for trimming and semi-thin sectioning of tissue blocks for electron microscopy

Answer 28

glass knives

Question 29

microtome knife used to cut any type of resin block for electron microscopy

Answer 29

diamond knives

Question 30

angle formed between the cutting edges

Answer 30

bevel angle

Question 31

bevel angle

Answer 31

27-32º

Question 32

theoretically, the perfect and optimum cutting angle

Answer 32

wedge angle

Question 33

angle with maximum penetration of tissues and minimizing distortion

Answer 33

wedge angle

Question 34

wedge angle

Answer 34

15º

Question 35

inclination of cutting plane to avoid compression of the block

Answer 35

clearance angle

Question 36

prevent uneven sections, or alternate thin and thick sections

Answer 36

clearance angle

Question 37

clearance angle

Answer 37

5-10º

Question 38

For the removal of gross Smooth stones, Machine hone Soapy water, Oil (Mineral oil, Castor oil, Clove oil), Xylene, Liquid Paraffin Heel-to-Toe Zigzag

Answer 38

honing (hard sharpening)

Question 39

For the removal of irregularities/blurr Shell horse leather Vegetable Oil Toe-to-Heel Zigzag

Answer 39

stropping

Question 40

water bath temperature

Answer 40

45-50ºC temperature should be between 5-10ºC below the melting point of the paraffin wax (56-65ºC)

Question 41

done to flatten the sections and prepare them for mounting

Answer 41

floating out/fishing out (water bath)

Question 42

done to fix the tissues into the slides. may use incubator, paraffin oven, hot plate.

Answer 42

drying

Question 43

to remove paraffin and render the tissue penetrable by the stain. done by immersing the slides into xylol (descending grades of alcohol)

Answer 43

deparaffinization