Stem cells + plant stem cells Flashcards

1
Q

what are the features of a stem cell

A

undifferentiated cells that continually divide and become more specialised
give rise to specialised cells
capable of self renewal
no limit to division
can be:
totipotent
pluripotent
multipotent

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2
Q

features of a totipotent stem cell

A

give rise to every specialised cell in the body (all 216)
undifferentiated/unspecialised cells
can keep dividing
form 8 embryo cell
all genes are switched on

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3
Q

features of pluripotent stem cells

A

give rise to most cells in the specialised cells in the body (except totipotent embryonic cells)
undifferentiated/unspecialised cells
no limit do division
from blastocyst or umbilical cord blood
some genes switched off

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4
Q

features of multipotent stem cells

A

undifferentiated/unspecialised cells
give rise to only those of closely related family
found from birth onward (e.g. bone marrow)
many genes switched off as cells have become differentiated

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5
Q

where are totipotent stem cells found

A

from the zygote
until the 8 cell embryo
all cells are totipotent since they have the potential to develop into all body cells

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6
Q

where are pluripotent stem cells found

A

5 days after fertilisation the blastocyte forms
outer blastocyte cell layer will form the placenta
inner cell mass will eventually form the embryo
source of pluripotent stem cells till day 16

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7
Q

where are multipotent stem cells found

A

after birth
found in bone marrow and body tissue

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8
Q

how do cells become specialised

A

some genes become switched on and some become switched off
switched on genes are transcribed and translated into proteins which are then used for specific function of that cell

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9
Q

what potency do plant cells remain there whole life and why is this important

A

remain totipotent
important since it allows plants to be grown from a fragment (explant) of a leaf, stem, root or from a single cell by plant tissue culture

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10
Q

how does plant tissue culture work

A

explant (fragment) of plant tissue is placed on sterile agar which contains growth regulators and nutrients using an aseptic technique
explants are then placed under a light bank for around 4 week
cells then differentiate to form a whole new plant
new plant is a genetically identical clone of the original plant

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11
Q

what are plant tissue cultures used for

A

improve plant and food crop species (e.g. create disease resistant plants)
produce increased quantities of plant chemicals used in drugs
produce commercial plants that are difficult to grow from seeds
conserve endangered plants

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