stem cells Flashcards
what are stem cells?
a stem cell is an unspecialised cell from the embryo, fetus or adult, that…
- under certain conditions, has self-renewal capability by reproducing itself for long periods
- can differentiate whereby unspecialised cells give rise to specialised cells that make up the tissues and organs of the body
- possess large nuclear to cytoplasmic ratio
stem cells are able to replenish their own population and generate cells that travel down various differentiation/developmental pathways
what are the 3 unique features of ALL stem cells?
- stem cells are unspecialised ⮕ stem cells do not have any tissue-specific structures that allows it to perform specialised functions, however unspecialised stem cells can give rise to specialised cells through specialisation/differentiation
- stem cells are capable of dividing and renewing themselves for long periods ⮕ stem cells may replicate many times in a process called proliferation. if resulting cells continue to be unspecialised, the cells are said to be capable of long-term self renewal by mitotic divisions
- stem cells can give rise to specialised cells. ⮕ internal (genes become activated & inactivated in a regulated way) & external (chemicals secreted by other cells, physical contact with neighbouring cells etc) signals trigger stem cell differentiation.
what is stem cell potency?
stem cell potency refers to the range of cell types to which a stem cell an give rise to.
it reflects the differential potential of the stem cell
it is determined by the number of pathways it can take in its subsequent development through specialisation/differentation
what are the 3 basic measures of stem cell potency?
- totipotent
- pluripotent
- multipotent
what are totipotent stem cells?
- ☆ totipotent stem cells are master cells of the body that have the ability to give rise to all cell types that make up an organism
- only zygotic stem cells are totipotent.
- totipotent stem cells have the potential to differentiate into any cell type in the adult body to form whole organisms AND any cell of the extra-embryonic membranes
- totipotent stem cells occur at the earliest stage of embryonic development, before the blastocyst stage
what are pluripotent stem cells?
- ☆ pluripotent stem cells descend from totipotent stem cells and have the ability to give rise to all types of cells that develop from the three germ layers - mesoderm, endoderm and ectoderm
- they do not have the potential to make differentiated cells that form the extra-embryonic membranes (unlike totipotent stem cells)
- embryonic stem cells (ESCs) are pluripotent stem cells, formed 5-7 days after fertilization
the only known sources of human pluripotent stem cells are those isolated and cultured from inner cell mass of early human embryos and from fetal tissue that was destined to be part of the gonads
what are multipotent stem cells?
- ☆ multipotent stem cells are cells that descend from pluripotent stem cells and can differentiate into a limited number of cell types OR many cell lines within a specific type of tissue
- more specialised than totipotent & pluripotent stem cells.
- formed after 7 days upon fertilisation
- blood stem cells are multipotent stem cells from the bone marrow give rise to all the cells of the blood (eg RBC, WBC, platelets), but not to other types of cells
- multipotent stem cells are found in most organs in adult animals, where they can replace dead or damaged cells
what are committed stem cells?
- ☆ committed stem cells (includes multipotent & unipotent cells) are those that have a more limited pathway of development compared to pluripotent cells and are destined to produce a specific group of cells
- once committed, stem cells do not switch commitment
- eg the hemangioblast stem cell gives rise to all the blood vessels, blood cells and lymphocytes
- committed stem cells can give rise to more specifically committed stem cells or can generate progenitor cells
what are progenitor cells?
- ☆ progenitor/precursor cells are intermediate cell types generated by stem cells before they achieve their fully differentiated state. progenitor cells are partly differentiated cells that divide and give rise to differentiated cells.
- progenitor cells usually show some evidence of differentiation, although the process is not complete until the fully differentiated cell has been formed
- progenitor cells are committed to a limited number of pathways of differentiation/development
- progenitor cells are more limited in developmental pathways than a multipotent stem cell
progenitor cells are derived from committed multipotent cells
what is the significance of mitotic division of stem cells?
bro idk how to phrase this lol
mitotic division of stem cells preserves a population of undifferentiated cells while steadily producing a stream of differentiating cells
what are the differences between embryonic stem cells and adult stem cells?
- potency ⮕ embryonic stem cells are pluripotent while adult stem cells are multipotent
- ability to culture in lab ⮕ embryonic stem cells are easier to culture compared to adult stem cells
- ethical issues ⮕ adult stem cells are less ethically problematic as not destruction of blastocysts are involved, compared to embryonic stem cells
- potential to differentiate ⮕ embryonic stem cells can become most cells/tissues of the body but most adult stem cells are limited to become specific types of tissue
- use for therapy ⮕ adult stem cells are already used in therapies (eg bone marrow transplant) while embryonic stem cells have great potential for developing future therapies
what are the 3 categories of embryonic stem cells?
- embryonic stem cells (ESCs) ⮕ can be isolated from the inner cell mass (ICM) of the blastocyst
- embryonic germ cells ⮕ can be isolated from the precursor to the gonads in aborted fetuses
- embryonic carcinoma cells (FYI)
what are the 6 defining properties of embryonic stem cells?
- ESCs are capable of undergoing an unlimited number of symmetrical divisions without differentiating
- ESCs exhibit & maintain a stable, full (diploid), normal complement of chromosomes
- ESCs are clonogenic, meaning a single ESC can give rise to a colony of genetically identical cells
- pluripotent ESCs can give rise to differentiated cell types that are derived from all 3 primary germ layers (endoderm, ectoderm, mesoderm)
- ESCs are capable of developing into all fetal tissues during development
- ESCs are easy to obtain pure and can be cultivated in large numbers, unlike adult stem cells
properties 4, 5 & 6 are unique to ESCs
what are primary roles of adult stem cells?
- maintain & repair the tissue in which they are found
- replace cells that die because of injury or disease
what is an adult stem cell?
- ☆ an adult stem cell is an undifferentiated cell found amongst differentiated cells in a tissue or organ, and can differentiate to yield the major specialised cell types of the tissue or organ
- adult stem cells are rare. they are dispersed in tissues throughout the mature animal and behave very differently, depending on their local environment. at any time, stem cell numbers in the body are very low, making them very difficult to identify & purify
- the origin of adult stem cells in mature tissues is unknown