photosynthesis Flashcards
what is ATP and describe its structure
ATP is adenosine triphosphate. ATP is made up of an adenine, a ribose and three phosphate groups.
how is ATP converted to ADP?
- the high-energy phosphoanhydride bonds carry enough energy to cause almost any reaction to proceed.
- chemical potential energy is quickly released to the organism or cell by a hydrolytic reaction which breaks the phosphoanhydride bond and converts ATP to ADP.
- this releases large amounts of energy for reactions to occur
- conversely, a phosphate can be reattached to ADP (phosphorylation) with the absorption of a large amount of energy, forming the high-energy phosphoanhydride bonds in ATP
what are autotrophs?
autotrophs are organisms that use an inorganic form of carbon, such as carbon dioxide, as starting materials for the synthesis of complex organic compounds. they are producers of the biosphere.
photoautotrophs are autotrophs which synthesise sugars from carbon dioxide and water using sunlight as the source of energy and chlorophyll for trapping the light energy. this process is called photosynthesis. photosynthesis is one of the most important biochemical processes because almost all living things depend on photosynthesis, directly or indirectly, for their organic compounds
why is photosynthesis important?
- it is how solar energy is captured by plants for use by all organisms
- it provides a source of complex organic molecules for heterotrophic organisms (organisms that cannot make their own food)
- it releases oxygen as a by-product for use by aerobic organisms
idk if this in syllabus but its not in LOs
what are the 3 main cell types in ground tissue?
3 main tissue types: dermal, vascular & ground
- parenchyma - living cells that carry out metabolic functions, including synthesis & storage of food. the cells are approximately spherical, with a thin cellulose primary cell wall. most common cell type, found in stems, roots and fleshy tissue of fruits.
- collenchyma - living cells that are specialised for flexible structural support. the cells are elongated with an unevenly thickened cellulose cell wall which are thicker than parenchyma cell walls. usually found just below epidermis and/or surrounding vascular bundles
- sclerenchyma - dead cells specialised for structural support. the cells are elongated and have evenly thickened cell walls that are thicker than collenchyma cell walls. cell walls contain lignin, which is impermeable to water, allowing specialised sclerenchyma cells to transport water. mature cells are found in parts of the plant which have stopped growing.
what cells in the leaf can chloroplasts be found in?
- palisade mesophyll cells
- spongy mesophyll cells
- stomata guard cells
what are the functions of each structural component of the leaf?
- upper epidermis - one cell thick, does not contain chloroplasts. the cells in the upper epidermis are quite transparent, allowing light to pass through to the photosynthetic tissue below.
- palisade mesophyll - the cytoplasms of palisade cells is full of chloroplasts. the number & arrangement of the chloroplasts, as well as the cell shape, allows maximum light capture.
- spongy mesophyll - the spongy mesophyll layer contains fewer cells than the palisade mesophyll. each cell has fewer chloroplasts so the cells are not as active in photosynthesis as the palisade. there are large intercellular spaces for the diffusion of CO2
- lower epidermis - the lower epidermis has stomata which are pores surrounded by a pair of guard cells. the only cells with chloroplasts in the lower epidermis are guard cells
what are the structural features of chloroplasts and their respective functions?
chloroplasts themselves are lens-shaped
chloroplast envelope
- made up of a double membrane
- outer membrane is selectively permeable to some solutes
- inner membrane is highly permeable and substances pass through with the aid of transporters
stroma
- stroma is the gel-like matrix enclosed by the chloroplast envelope
- contains circular DNA, 70S ribosomes, starch granules, oil droplets and enzymes involved in the calvin cycle
thylakoids
- a third membrane system within the stroma consisting of flattened membranous sacs or pouches
- photosynthetic pigments and electron carriers are embedded within the membrane
- the space enclosed within the thylakoid is known as the thylakoid lumen or thylakoid space
- this compartmentalisation allows chemiosmosis to take place and for ATP to be produced by photophosphorylation
granum
- a stack of thylakoids
- increases surface area and amount of pigments available for the light-dependent reaction of photosynthesis
- intergranal lamellae (singular: lamella), which are flattened membranous tubular thylakoids, connect the grana. these lamellae connect the thylakoid compartments into a single, continuous compartment within the stroma.
(read through) understanding light
- light is a fom of electromagnetic energy. light with different amounts of energy have different wavelengths. the entire range is called the electromagnetic spectrum.
- visible light is the range of wavelengths that can be detected by the human eye
- when light meets matter, it may be reflected, transmitted or absorbed
- pigments are chemical compounds which reflect only certain wavelengths of visible light. pigments are also able to absorb only certain wavelengths of light. the pigment molecules act like energy-receiving antennas to capture light energy for photosynthesis
- each pigment absorbs wavelength of a narrow range within the spectrum -> plants usually need to use several kinds of pigment to effectively increase the range of wavelengths from which they can obtain energy
what are the two basic classes of photosynthetic pigments in plants?
- chlorophyll (main & most abundant pigment) & carotenoids (accessory pigment, incuding carotenes & xanthophylls)
- both chlorophyll & carotenoids are found in the thylakoid membrane of chloroplasts
- examples of chlorophyll pigments: chlorophyll a (yellow-green) and chlorophyll b (blue-green)
- examples of carotenoid pigments: b-carotene (orange) and xantophylls (all yellow)
describe the structure and function of chlorophyll
- chlorophyll is the main pigment utilised in photosynthesis
- chlorophyll absorbs mainly red and blue-violet light. it reflects green light, giving most plants their characteristic green colour.
- each molecule of chlorophyll consists of a hydrophilic porphyrin ring and a hydrophobic hydrocarbon tail
- the hydrophilic porphyrin ring functions in light absorption. it has a flat, light-absorbing hydrophilic head which contains a magnesium atom at its centre. magnesium deficiency in plants reduces chlorophyll production and causes yellowing (chlorosis)
- the hydrophobic hydrocarbon tail projects into the thylakoid membrane, keeping the chlorophyll embedded in the thylakoid membrane
- different chlorophylls have different side chains on their hydrophilic head -> modifies their absorption spectra & increases the range of wavelengths of light absorbed.
- chlorophyll a is the major pigment in autotrophs, absorbing blue & red light. only chlorophyll a participates directly in the light-dependent reaction. other pigments (aka accessory pigments) can absorb light and transfer the energy to chlorophyll a. chlorophyll b is themost common accessory pigment
- chlorophyll is always associated with specific binding proteins, forming light-harvesting complexes (LHCs) in the thylakoid membrane
describe the structure and function of carotenoids
- carotenoids are accessory pigments, as they pass the light energy they absorb onto chlorophyll a of the reaction centre.
- carotenoids are yellow, orange, red or brown pigments that absorb strongly in the blue-violet range
- carotene & xanthophyll both absorb light in the 460-550nm of the visible light spectrum. so by using both carotenoids and chlorophyll, efficiency of light-harvesting is increased as the range of wavelengths that can be captured is increased.
- carotenoids & other accessory pigments serve 2 main function: broadening the spectrum of light for photosynthesis and photoprotection
- broadening spectrum of light for photosynthesis: accessory pigments absorb the intermediate wavelengths of light which chlorophyll cannot, broadening the spectrum of colours that can drive photosynthesis. but carotenoids transfer only about 10% of their absorbed energy, and are not very effective as a photosynthetic pigment
- photoprotection: carotenoids are more important in absorbing excessive light & preventing auto-oxidation of chlorophyll, hence preventing photobleaching. this is known as photoprotection. excessive light intensity can damage chlorophyll pigments, so instead of transmitting energy to the chlorophyll, some carotenoids absorb & dissipate excess light energy from chlorophyll, protecting them from destruction by light.
PLS LOOK AT THE DIAGRAMS
★ what is the absorption spectrum & action spectrum?
PLS LOOK AT THE DIAGRAMS
- the absorption spectrum of a photosynthetic pigment is a graph of the amount of light absorbed at different wavelengths by a pigment
- the action spectrum for photosynthesis is a graph of the effectiveness of different wavelengths of light in driving photosynthesis
- both spectrums show that the wavelengths of light absorbed by chlorophyll, red & blue light, are very similar to the wavelengths that drive photosynthesis, so chlorophyll is mainly responsible for the absorption of light in photosynthesis
- both spectrums also show that the wavelengths absorbed are the ones that provide most energy for photosynthesis, so both blue and red light are used by plants as the energy source for photosynthesis
what are the 3 main stages of photosynthesis?
- light harvesting
- light dependent reaction
- light independent reaction
what is excitation of chlorophyll by light?
when a chlorophyll or some other photosynthetic pigment absorbs light, it changes from its ground state to excited state.
the excited molecule is unstable and tends to return to its original, ground state in 1 of 3 ways:
1. by transferring the energy (NOT the electron) directly to a neighbouring chlorophyll molecule by a process called resonance energy transfer. this occurs during light harvesting
2. by boosting an electron to a higher energy level and then transferring it to an electron acceptor (a nearby molecule capable of accepting electrons). the molecule returns to its original state by taking up a low-energy electron from another molecule (electron donor). this occurs in the light-dependent reaction.
3. (not used in photosynthesis) energy is lost in the process. this is achieved by converting excecss wat into heat or to a combination of heat and light to a longer wavelength. this occurs when light energy is absorbed by an isolated chlorophyll molecule in solution.