Stem Cell Biomanufacturing Flashcards
1
Q
Hematopoietic Stem Cells
A
- can self-renew
- used in HSC transplants
- BM, peripheral blood or umbilical cord blood
2
Q
Why HSC transplant ?
A
- reconstitutes BM
- restores normal hematopoiesis after chemotherapy
3
Q
HSCT: Graft sources
A
- syngeneic (identical)
- allogeneic (sibling, matched)
- autologous
4
Q
Autologous Transplant Process
A
- Collection (peripheral or BM)
- Processing
- purified + concentrated - Cryopreservation
- Reinfusion
- thawed stem cells returned to patients
5
Q
Lab Responsibilities for Autologous Transplant
A
- stem cell quality assessment
- processing
- cryopreservation
- storage
- distribution
6
Q
HSC Processing
A
- volume weighed (can reduce amount of plasma = concentrated)
- Cryoprotectant (controlled process to prevent cell damage)
- ## Cryopreservation & Storage
7
Q
Assays for HSC Product Characterization (2)
A
- Flow Cytometry: HSC count and Viability
- CFU: Stem Cell Potency (proliferative potential)
7
Q
HSC Product Characterization
A
- WBC/ kg
- CD34+/ kg
- CFU/ kg
- cell viability
- infectious diseases
- sterility
8
Q
HSC Cryopreservation
A
- specific cooling profiles (reduce ice formation)
- stored less than -80°C, indefinitely
9
Q
How long it takes for HSC function after transplant
A
2-3 weeks
10
Q
HSC Post-transplant Care
A
- routine charting
- monitor HSC engraftment (ANCs and PLT as indicators)
- monitor adverse rxns
ANCs - absolute neutrophil count
11
Q
Cord Blood HSC
A
- mother as donor (consent, ABO/Rh)
- ex utero collection
12
Q
Pros vs Cons of Cord Blood HSC
A
Pros:
- lower GvHD (mismatch okay)
- higher [HSC]
- lower viral transmission
Cons:
- limited cell volume
