Steinhauer Flashcards

1
Q

P. falciparum

A

Mostly tropics
30,000 merozoites from hepatic cycle
Like younger cells
Only see multiple ring form and gametocytes (banana shaped)
Only one to do the clumping and rosetting

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2
Q

P. vivax

A
Tropics but not that much in africa
10,000 merozoites per hepatic cycle
Like reticulocytes
Can relapse
See schizont and macrogametocytes
One ring
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3
Q

P. malariae

A

Cosmopolitan but spotty

Like older RBC

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4
Q

P. ovale

A

Can relapse

Likes young

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5
Q

P. knowlsi

A

Southeast asia

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6
Q

Anopheles

A

Face down, ass up, thats the way it likes to suck

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7
Q

Plasmodium life cycle

A

Mesquito injects sporozoits into human
Make it to liver by inducing parasitophorous vacuole
The replicate and release merozoites back into blood
Make it to blood cells and make trophozoites

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8
Q

Ring troph

A

Signet ring trophozoite
Hemozoin with schizont
Can reenter cycle or make micro/macrogametocyte which get picked up by mesquito

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9
Q

How long until you have malaria symptoms

A

14-35 days

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10
Q

Uncompliccated malaria

A
Acute fever
Paroxysms- chill then sweats
Vivax every 48 hours benign tertian
Falciparum every 72 hours malignant tertian
Due to TNF-a
Anemia
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11
Q

Complicated malaria

A

Organ failure
Cerebral- Neuro problems
Blackwater fever- Dark hemoglobinuria
Hyperparasittemia with 5% RBC infected

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12
Q

Why is P. falciparum more pathogenic

A

More merozoites, more invasion of RBC
PfEMP1- expression causes cytoadhesions=endothelium adhesions and rosetting and protects parasites from being cleared by spleen

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13
Q

Sticky cells and disease

A

TNF—iNOS which disrupts neurons- cerebral effects

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14
Q

Malarial relapse

A

Normally within 3-5 years
Have a dormant state in liver with clearance from RBC
P. vivax and ovale

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15
Q

Recrudescense

A

Low level in RBC with no hypnozoites

P. Falciparum

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16
Q

Plasmodium dx

A

Thick blood smear- recognize parasite
Thin blood smear- specific dx
Serological, PCR
Rapid- for falciparum (HRP2) and vivax

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17
Q

Genetic resistance to malaria

A

Sickle cell (PfEMP1 malformation, microRNA also disrupt parasite, and heme oxygenase 1 inhibits cerebral malaria)
Duffy blood groups (vivax and knowlesi)
Thalassemia
G6PDH

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18
Q

Duffy blood group

A

Genes common in africa, the reason why vivax doesn’t exist there
Lack of receptors for parasite attachment

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19
Q

Host defense of plasmodium

A

Spleen clears infected cells

Ab- Premunition: asymptomatic parasitemia (not common with travelers)

20
Q

Babesia microti

A

Northeastern and upper midwest

21
Q

Babesia divergens

A

Midwest and europe

22
Q

Babesio duncani

A

West coast

23
Q

Babesiosis general

A

Flu like symptoms unless immunocomprimised
Most via tick (Ixode scapularis and pacificus), some from blood transfusion
Important host- Permyscu leucopis mouse, same as Borrelia

24
Q

Babesiosis life cycle

A

After feeding, adult females drop off the third host to lay eggs(1), usually in the fall. Eggs hatch into six-legged larvae(2)and overwinter in the larval stage. In the spring, the larvae seek out and attach to the first host, usually a small rodent(3). Later in the summer, engorged larvae leave the first host(4)and molt into nymphs(5), usually in the fall. The ticks overwinter in this stage. During the following spring, the nymphs seek out and attach to the second host(6), usually another rodent (e.g., a mouse) or lagomorph (e.g., a rabbit). The nymphs feed on the second host and drop off later in the summer(7). Nymphs molt into adults(7a-7b)off the host in the late summer or fall, and overwinter in this stage. The next spring, adults seek out and attach to a third host, which is usually a larger herbivore (including cervids and bovids), carnivore, or human(8). The adults feed and mate on the third host during the summer. Females drop off the host in the fall to continue the cycle. Females may reattach and feed multiple times. The three hosts do not necessarily have to be different species, or even different individuals. Humans may serve as first, second or third hosts.

25
Babesia microti life cycle
The Babesia microti life cycle involves two hosts, which includes a rodent, primarily the white-footed mouse, Peromyscus leucopus, and a tick in the genus, Ixodes.  During a blood meal, aBabesia-infected tick introduces sporozoites into the mouse host .  Sporozoites enter erythrocytes and undergo asexual reproduction (budding) .  In the blood, some parasites differentiate into male and female gametes although these cannot be distinguished at the light microscope level .  The definitive host is the tick.  Once ingested by an appropriate tick , gametes unite and undergo a sporogonic cycle resulting in sporozoites .  Transovarial transmission (also known as vertical, or hereditary, transmission) has been documented for “large”Babesia spp. but not for the “small” babesiae, such as B. microti . Humans enter the cycle when bitten by infected ticks.  During a blood meal, a Babesia-infected tick introduces sporozoites into the human host .  Sporozoites enter erythrocytes  and undergo asexual replication (budding) .  Multiplication of the blood stage parasites is responsible for the clinical manifestations of the disease.  Humans are, for all practical purposes, dead-end hosts and there is probably little, if any, subsequent transmission that occurs from ticks feeding on infected persons.  However, human to human transmission is well recognized to occur through blood transfusions .
26
Babesia dx
No hemozoin (may not see with falciparum) Ring form can make tetrad/ maltese cross Can have multiple rings per cell Irregular circles
27
Pathogenesis of Babesia
``` Serologic testing IFA (ELIZA for B. microti) Tick- 1-6 wks incubation Symptoms: Intermittent fever, dark urine 1-20% RBC normally infected Summer transmission ```
28
Erlichiosis chaffeensis
``` Rickettsial tick borne Intracellular G- Human monocytic ehlichiosis Morula in monocyte Amblyomma vector, deer reservoir Gets worse with age South midatlantic, north/south Can get rash everywhere (also hands/feet) ```
29
Anaplasmosis phagocytophilum
``` Rickettsial tick borne Intracellular G- Human granulocytic anaplasmosis Morula in granulocytes Ixodes scapularis and pacificus vector and white footed mouse reservoir Mostly Eastern states, more north Gets worse with age ```
30
Erlichiosis symptoms
1 wk incubation fever, chlls, headache, myalgia, malaise Ambylomma americanum tick vector
31
Anaplasmosis
1-2 week incubation fever, chlls, headache, myalgia, malaise Ixode vector
32
Ixode vector pathogens
Babesia, anaplasma, and borrelia burgdorferi
33
How to treat Ehrlichiosis, anaplasmosis, and early stage lyme disease
Doxycycline
34
Babesia tx
Clinda and quinine
35
Dermocentor vector pathogen
Erlichia, Ricketsia, Tularemia
36
Soft tick vector
Borrellia recurrentis
37
Herpes replication
Linear DNA, circular during infection | Rolling circle replication
38
EBV
``` Mono with heterophiles Nasopharyngial carcinoma Burkitt’s lymphoma (Higher if with malaria) Hodgkin lymphoma Gastric carcinoma Kissing disease CR2 (a/k/a CD21), on susceptible cells Activates B cells= LN swelling Activates CTLs (atipical lymphocytes) ```
39
Mono
Pharyngitis Hepatosplenomegaly (3-4 weeks) Lyphadenopathy atypical lymphocytes
40
CMV
``` Mono Pneumonia Retinitis, esophagitis in immunocompremised Monospot negative Owl eye in histology Exudate tonsillitis more rare!!! Dx: Histo and PCR ``` Evade CD8 cells by inhibiting MHC1 expression
41
Ampicillin rash
Caused when heterophile Abs are present | EBV
42
HIV
``` GALT important Symptoms like MONO Thrush and rash as well +ssRNA diploid with env LTR- 5' RNA transcript, 3' term/poly/int gp120 (adhere) gp41(fusion) ENV Gag, Pro, Pol, Env 2-4 weeks for symptoms ```
43
HIV testing
1/2nd gen- IgG test 3rd gen- IgM test 4th- Can test for IgM or Ag
44
Pneumocystis pneumonia
``` Dry cough, low fever, dyspnea with hypoxia Butterfly chest xray (bilateral interstitial) Elevated LDH (over 500mg/dl) Consider CMV and pneumococci Do Bronchoaveolar lavage (BAL) to rule these out. Stain + with silver Monitor kidney function with tx DDx-Streptococcal pneumonia Tuberculosis Disseminated MAC Tx- TMP-SMZ for 21 days Steroids for airway inflammation control ```
45
Candidiasis
Taste disturbance, sometimes burning Erythematous mucosa C. Esophagitis- dysphagia, retrosternal pain, nauseau Do not confuse with Oral Hairy Leukoplakia (OHL) related to EBV Consider Alcoholism and steroid tx Tx- Oral 7 days, esophageal 14-21 days of fluconazole
46
Atypical mycobacterioses
``` MAC (M Avium) Elevated Alk. Phos Try to culture sputum Bone marrow/lymph for confirmation CD4 <50, use prophylaxis to prevent dissem. Diss.- Multi organ, liver more common DDx- TB or malignant lymphoma ``` preferred: clarithromycin + ethambutol alternative to clarithromycin: azithromycin Use for more than 12 months
47
Cryptococcosis
``` Southeast asia, and US Neuro problems with increased ICP, do LP Atipical pneumonia/ chest pain/unproductive cough Photophobia and low grade fever Extensive abscesses Lesions resemble molluscum cantagiosum Tx- Amphotericin B and flucytosine (induction phase) monitor renal fx Fluconazole (consolidation phase) 400mg Fluconazole 200mg (maintenance) ``` + for india ink, antigen good for Dx