Stains Memoriser

Question 1

SCC

Answer 1

Cytokeratins AE1/AE3, 34BE12, MNF116, 5/6, EMA, Ki-67

Question 2

Paget’s disease of the breast

Answer 2

CAM 5.2, CK7, CEA

Question 3

Non-gastric adenocarcinoma

Answer 3

CK7

Question 4

Merkel cell carcinoma

Answer 4

CK20 (perinuclear dot); Synaptophysin, Chromogranin, Neuron-specific enolase
TTF-1 NEGATIVE

Question 5

Metastatic adenocarcinoma

Answer 5

CDX2, CK7, CK20 (CK7/20 can be useful to identify location, among other stains)

Question 6

Sebaceous carcinoma

Answer 6

EMA, adipophilin

Question 7

Epithelioid sarcoma

Answer 7

EMA

Question 8

Adnexal neoplasms

Answer 8

CEA

Question 9

BCC

Answer 9

BerEp4 (negative EMA)

Question 10

Metastatic renal cell carcinoma

Answer 10

RCC

Question 11

Metastatic small-cell lung carcinoma

Answer 11

TTF-1 (as compared with MCC, which is positive)

Question 12

Desmoplastic melanoma

Answer 12

S100, Sox10

Question 13

Spitz naevi

Answer 13

S100A6

Question 14

Cellular neurothekeoma

Answer 14

S100A6, MITF

Question 15

Langerhans cell histiocytosis

Answer 15

S100, CD1a

Question 16

Rosai-dorfman disease

Answer 16

S100

Question 17

Granular cell tumour

Answer 17

S100

Question 18

Neurofibroma

Answer 18

S100

Question 19

AFX

Answer 19

S100A6

Question 20

Naevi

Answer 20

Melan-A/MART-1

Question 21

Melanoma

Answer 21

S100, Melan-A/MART-1, Sox10, MITF

Question 22

Schwannoma

Answer 22

Sox10

Question 23

Sarcoma

Answer 23

Vimentin

Question 24

Piloleiomyoma

Answer 24

Desmin, SMA

Question 25

Leiomyosarcoma

Answer 25

Desmin

Question 26

Glomus tumour

Answer 26

SMA

Question 27

Glomangioma

Answer 27

SMA

Question 28

DFSP

Answer 28

CD34

Question 29

Angiosarcoma

Answer 29

CD31

Question 30

Infantile haemangioma

Answer 30

GLUT1

Question 31

Perineurioma

Answer 31

GLUT1

Question 32

Dermatofibroma

Answer 32

Factor XIIIa

Question 33

Lymphangioma

Answer 33

D2-40

Question 34

Lymphovascular invasion

Answer 34

D2-40

Question 35

B-cell lymphomas

Answer 35

CD19, CD20, CD79a

Question 36

T-cell lymphomas

Answer 36

CD3, CD4, CD8

Question 37

Mycoses fungoides

Answer 37

CD3, CD4, CD8, CD5 and 7 loss, CD30 with transformation

Question 38

CLL

Answer 38

CD5, CD23

Question 39

Mantle cell lymphoma

Answer 39

CD5, (CD 23 negative), Bcl2

Question 40

Anaplastic large-cell lymphoma

Answer 40

CD30

Question 41

Lymphomatoid papulosis

Answer 41

CD30

Question 42

Elastic plasmacytoid dendritic cell neoplasm

Answer 42

CD56

Question 43

Histiocytic markers

Answer 43

CD68, and CD163

Question 44

AML

Answer 44

Myeloperoxidase

Question 45

Multiple myeloma

Answer 45

CD138

Question 46

Urticaria pigmentosa

Answer 46

CD117 (c-Kit)

Question 47

Follicle centre cell lymphoma

Answer 47

Bcl6, CD10

Question 48

Diffuse large B-cell lymphoma, leg type

Answer 48

MUM-1

Question 49

Syphilis

Answer 49

Spirochete stain

Question 50

HSV

Answer 50

HSV stain

Question 51

VZV

Answer 51

VZV stain

Question 52

Kaposi's sarcoma

Answer 52

HHV-8 stain