Stains & Media Flashcards

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1
Q

Giemsa

A

differential stain

Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium
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2
Q

PAS

A

stains glycogen, mucopolysaccharides

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3
Q

Ziehl-Neelson (carbol fuschin)

A

acid-fast organisms

Nocardia
Mycobacterium

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4
Q

India Ink

A

capsule
Cryptococcus neoformans
(mucicarmine - red thick polysaccharide)

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5
Q

Silver Stain

A

fungi, Legionella, H. pylori

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6
Q

Haemophilus influenzae

A

chocolate agar with factors V (NAD+) and X (hematin)

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7
Q

Neisseria

A

thayer-martin/VPN media

vancomycin: inhibits Gr(+)
polymixin: inhibits Gr(-), except Neisseria
nystatin: inhibits fungi

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8
Q

Bordetella pertussis

A

bordet-gengou (potato) agar

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9
Q

Corynebacterium diphtheriae

A

Tellurite plate, Löffler’s media

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10
Q

Mycobacterium tuberculosis

A

Löwenstein-Jensen agar

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11
Q

Mycoplasma pneumoniae

A

Eaton’s agar

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12
Q

Lactose-fermenting enterics

A

pink colonies on MacConkey’s agar

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13
Q

Legionella

A

buffered charcoal yeast extract agar with cysteine and iron

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14
Q

Fungi

A

Sabouraud’s agar

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15
Q

MacConkey

A
isolates Gr(-)
bile salts inhibits Gr(+)
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16
Q

Vibrio cholerae

A

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Agar

alkaline media