stains and microscopy

Question 1

Sterheimer-malbin

Answer 1

delineates nucleus and cytoplasm
used for WBC, epi cells, and casts (purple)

Question 2

Toluidine blue

Answer 2

Nuclear detail
differentials WBC from RTE (blue)

Question 3

2% acetic acid

Answer 3

Enhances WBC nucleus
Differentials RBC from WBC, yeat, etc.
Dissolves amorphous crystals and WBC granules

Question 4

2% sodium hydroxide

Answer 4

Dissolve amorphous urates

Question 5

Lipid stains

Answer 5

stains triglycerides and neutral fats
Does not ID cholesterol
Reddish color

Question 6

Gram Stain

Answer 6

Stains bacteria
IDs bacterial casts (red or purple)

Question 7

Hansel Stain

Answer 7

Eosinophils from other WBCs
reddish stain

Question 8

Prussian blue

Answer 8

Structures containing iron
ID hemociderin (blue)

Question 9

Bright field microscopy

Answer 9

Routine urinalysis

Question 10

Phase contrast

Answer 10

Enhances low reflective objects (hyaline casts, mucus, etc)

Question 11

Polarizing microscopy

Answer 11

Used for cholesterol and crystals

Question 12

Interference contrast

Answer 12

enhances detail (3D image)