Stains Flashcards
What is staining?
A biochemical technique of adding a specific dye to a substrate (DNA, proteins, lipids) to qualify or quantify the presence of a specific compound.
Who invented Romanowsky stains and when?
Buitri Leonovich Romanowsky in the year 1891.
What are the components of the original Romanowsky stain?
Water soluble stain eosin and methylene blue.
What are the two main components of the Romanowsky effect?
Azure B (trimethylthionine) and Eosin Y (tetrabromofluorescein).
What type of molecules does Azure B bind to?
Anionic molecules.
What type of sites does Eosin Y bind to?
Cationic sites on proteins.
What is the recommended pH for general use of Romanowsky stains?
6.8.
List at least three stains included under Romanowsky stains.
- May-Grunwald stain
- Jenner stain
- Giemsa stain
- Leishman stain
- Wright stain
- Field stain
What is the purpose of Leishman stain?
It’s a combined fixative and staining solution.
Fill in the blank: For Leishman stain, after flooding the slide with stain, drops of water are added to ________ the film.
double dilute
What color does the nucleus stain with Leishman stain?
Purple.
What color does erythrocyte stain with Leishman stain?
Dark pink.
What color does lymphocyte stain with Leishman stain?
Blue.
True or False: Eosinophilic granules stain strongly with the basic component of the dye.
False.
What color do neutrophil granules stain with Romanowsky stains?
Pink/orange.
What color do promyelocyte primary granules stain?
Red/purple.
What color do basophilic granules stain?
Purple-black.
What is the staining result for toxic granules?
Dark purple.
What color do platelets stain with Romanowsky stains?
Purple.
