Staining Tissue Preseravtion

Question 1

What is Staining ?

Answer 1

Is a technique used in microscope enhance contrast in microscopic image .

Question 2

Dyes

Answer 2

Are used specifically whenever a cell including animal and plant material have been visualized

Question 3

Microscopy Dyes

Answer 3

Used mainly in histology, cytology and microbiology but also in analysing textile fibres , paper and other technical products

Question 4

What is Vivo Staining ?

Answer 4

Is the process of dyeing living tissues vivo means in life which is compared with vitro staining .

Question 5

How does it work? Vivo staining

Answer 5

By letting certain cells or structures to taken on contrasting colours their form or positions within a cell or tissue

Question 6

What is the purpose ?

Answer 6

Is to reveal cytolical details which is not apparnat

It can however reveal to us where certain chemical reations can take place within the cells or tissue.

Question 7

Vitro Staining

Answer 7

Involves coluring cells or structures which are not living.

Certain features are merged or combines to increase the detail and features rather than a single stain

They are combined with specific protocols for fixation and sample preparation ,scientist and physians can use these standard techniques as consistent and repeatable diagnostic

Counterstain is stain which makes cells or structures more visible than when its not completely visible .

Question 8

Procedures in Preparing a Sample

Answer 8

1. Permeabilization this is to do with the treatments of cells with a mild surfactant which dissolves cell membranes to allow larger dyes moclues to enter inside.
2. Fixation : Serves to fix or preserve cells or tissues morphology through preparation process.

Involves adding a chemical fixation that creates chemical bonds between proteins to increase their rigidity

Common fixatives include formaldehyde , ethanol and methanol and or picric acid .