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HISTOPATH > Staining & Mounting > Flashcards

Staining & Mounting Flashcards

1
Q

Components are demostrated by DIRECT INTERACTION with Dye or staining solution

A

HISTOLOGICAL STAINING

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2
Q

Components are studied through chemical reaction; for demonstration of CHEMICAL COMPONENTS

A

Histochemical / Histochemistry staining

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3
Q

Histochemical staining used for HEMOGLOBIN

A

Pearl’s Prussian Blue reaction

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4
Q

Histochemical staining used for Carbohydrates

A

Periodic Acid Schiff

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5
Q

Demonstrate tissue ANTIGEN

A

Immunohistochemical staining

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6
Q

This is a combination of immunologic and histochemical staining

A

Imunnohistochemical staining

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7
Q

Detection of phenotypic markers that are detected by antibody

A

Immunohistochemical

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8
Q

Staining technique that uses aqueous or alcoholic dye to produce color

A

Direct Staining

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9
Q

Type of staining techniques that uses one dye and color of the dye is usually the resulting color

TISSUE + DYE = COLOR

A

Direct Staining

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10
Q

Type of staining that uses MORDANT or ACCENTUATOR to produce color

A

Indirect Staining

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11
Q

Substance that serves as the link/bridge between the dye and the tissue

A

MORDANT

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12
Q

EXAMPLES OF MORDANT

A

Erlich
Cole
Mayers
Gills
ALUM ( potassium alum)

Iron
- Heidenhain = ferric ammonium sulfate
- Weigerts = ferrim ammonium chloride

Copper = copper
PTAH = phophotungstic acid

ALL ARE HEMATOXYLINS

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13
Q

Substance that INCREASES you he color intensity and selectivity of the dye

A

ACCENTUATOR

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14
Q

EXAMPLES OF ACCENTUATOR

A

KOH
Phenol in carbol fuchsin and carbol thionine

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15
Q

Staining technique that use gradual application of dye. Tissue elements is re-stained in DEFINITE SEQUENCE

A

Progressive staining

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16
Q

Dye is applied little by little until desired color is achieved

A

Progressive staining

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17
Q

This staining technique do not need DECOLORIZATION

A

Progressive staining

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18
Q

Tissue is overstained and excess dye should be removed using DECOLORIZATION

A

Regressive staining

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19
Q

Selectively removal of excess of dye

A

DECOLONIZATION OR DIFFERENTIATION

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20
Q

This is the most common DECOLORIZATION substance and can remove both ACID and BASIC dye

A

ACID ALCOHOL

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21
Q

This of type of staining technique that involves application of different color to produce contrast and background

A

counterstaining

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22
Q

This of type of staining technique that involves application of different color to produce contrast and background

A

counterstaining

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23
Q

Type of staining technique that stained tissue with a color that is DIFFERENT from the stain itself

A

Metachromatic staining

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24
Q

Which stain is not belong to Metachromatic staining

Methyl violet
Cresyl blue
Safranin
Nile blue
Methylene blue

A

Nile blue

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25
Q

This staining technique stained tissue with the SAME SHADE /HUE as that if the dye

A

Orthochromatic staining

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26
Q

Type of staining technique that is Selective to living cells

A

Vital staining

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27
Q

Two types of Vital staining

A

Intravital & Supravital

28
Q

The dye/stain is so injected directly to any part of the LIVING BODY

A

Intravital

29
Q

Stains is applied immediately to the tissue after its REMOVAL from living body

A

Supravital

30
Q

Best vital stain

A

Neutral red

31
Q

Especially recommended for MITOCHONDRIA under supravital staining

A

Janus green

32
Q

type of staining technique that DO NOT USE DYE instead use METALLIC SALTS

A

Metallic IMPREGNATION

33
Q

Leaves black deposits on the surface of the tissue

A

Metallic impregnation

34
Q

Which of these metallic salt cannot be used?

Ammoniacal silver
Silver nitrate
Ammoniacal gold
Gold chloride

A

Ammoniacal gold

(There is no ammoniacal gold)

35
Q

Most common method that utilized MICROANATOMICAL STUDIES OF TISSUE

A

H&E Staining

36
Q

Dye used in cytoplasmic stain; counterstaining

A

EOSIN

37
Q

TRUE OR FALSE

tissue fixed with mercuric chloride staining time should be DECREASED slightly while duration of eosin should be INCREASED

A

False

staining time should be INCREASED
duration should be REDUCED

38
Q

H&E result color for Nuclei

A

Blue to blue black

39
Q

H&E color result for Karyosome

A

Dark blue

40
Q

H&E color result for Cytoplasm

A

Pale pink

41
Q

H&E color result for RBC and Eosinophilic granules

A

Keratin-bright orange red

42
Q

H&E color result for Calcium and decalcified bone

A

Purplish blue

43
Q

H&E color result for decalcified bone MATRIX, collagen and osteoid

A

PINK

44
Q

H&E color result for muscle fibers

A

Deep pink

45
Q

what are the staining methods for FROZEN section

A

H&E
Thionine
Polychrome methylene blue
Alcoholic pinacyanol method

46
Q

Derived from plants and animals

A

Natural Dyes

47
Q

Not a stain, active coloring agent is HEMATEIN

A

Hematoxylin

48
Q

Mordant of hematoxylin

A

Alum, iron, copper, chromium

49
Q

Hematoxylin mordant that is also a general for photomicrography

A

Iron hematoxylin

50
Q

Under alum hematoxylin, ripening agents for exfoliative cytology and demonstration of sex chromosome

A

Harris hematoxylin

51
Q

Under alum hematoxylin a ripening agent that use sodium iodate with citric acid & chloral hydrate as preservative for immunohistochemistry

A

Mayers hematoxylin

52
Q

Mordant of Weigert’s hematoxylin for muscle fibers and CT

A

Ferric ammonium CHLORIDE

53
Q

Mordant of Heidenhain’s hematoxylin for nuclei &cytoplasmic inclusion

A

Ferric ammonium SULFATE

54
Q

Demonstrate spermatogenesis

A

Copper hematoxylin

55
Q

Demonstrate endocrine cells of alimentary tract

A

Lead hematoxylin

56
Q

Demonstrate of collagen, reticulin and argentaffin cells

A

6 Molybdenum hematoxylin

57
Q

Extracted from bug (Coccus cacti)

A

Cochineal dye

58
Q

For neutropatholigical studies

A

Pictic acid picro carmine

59
Q

Natural dye for glycoge

A

Aluminum chloride best carmine

60
Q

Vegetable oil extracted from lichens used for staining ELASTIC FIBERS

A

Orcein

61
Q

Vegetable dye extracted from lichens but is NOT USED IN CYTOLOGICAL STAIN

A

Litmus

62
Q

Aka COAL TAR DYES / ANILINE DYES

A

Synthetic dyes

63
Q

Responsible for coloring property giving imparting color

A

Chromophore

64
Q

Responsible for dyeing property retaining imparted color

A

Auxochrome

65
Q

Without auxochrome component that demonstrates fats

A

Lysochrome

66
Q

Most sensitive of oil soluble dyes

A

Sudan black B

HISTOPATH (10 decks)

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