Special Stains (Carbohydrates)

Question 1

Stains Mucin

Answer 1

Best Carmine Method
Mucicarmine Stain

Question 2

Differentiates Acid Mucin from Neutral Mucin

Answer 2

Combined Alcian Blue-pas-hematoxylin Technique

Question 3

Stains Sulfated Mucins

Answer 3

Gamori’s Aldehyde

Question 4

Stain Acid Mucin

Answer 4

Colloidal Iron
Fluorescent Arcidine Orange

Question 5

Stains Encapsulated: C. Neoformans

Answer 5

Southgate’s Mucicarmine Technique

Question 6

Stains Glycoaminoglycans

Answer 6

Metachromatic Staining - Azure A
Metachromatic Toluindine Blue

Question 7

Best Carmine stains the following

Answer 7

Glycogen
Mast Cell Granules
FIBRIN
MUCIN

Question 8

Used for the demonstration of GLYCOGEN specially when it uses the Diastase Method; may also stains MUCOPROTEINS, MUCIN and PHOSPHOLIPIDS

Answer 8

Periodic Acid Schiff (PAS)

Question 9

Has a good affinity to glycogen (bright red) due to alkaline carminic acid

BUT weakly stains mast cell granules, fibrin, mucin

Answer 9

Best Carmine Method

Question 10

An OBSOLETE STAIN and NOT SPECIFIC for glycogen.

Rapid but tends to fade after few months

Answer 10

Langhan’s Iodine Method

Question 11

Stains Glycoaminoglycans and gives RED-PURPLE stains

Answer 11

Metachromatic Staining - Azure A
Metachromatic Toluidine Blue

Question 12

Most popular method for ACID MUCIN (+ blue )

Answer 12

Alcian BLUE Technique

Question 13

Differentiates Neutral Mucin from Acid Mucins

Answer 13

Combined Alcian Blue-PAS-Hematoxylin technique

PAS = Neutral Mucin
Alcian Blue = Acid Mucin

Question 14

Demonstrate mucin by Adding ALUMINUM HYDROXIDE to Carmine

Answer 14

Mucicarmine stain

Question 15

This stain carbohydrates and absorbed onto tissue containing ACID MUCIN at LOW pH and visualized by its conversation to FERRIC CYANIDE

Answer 15

Colloidal (Dialyzed) Iron Technique

Question 16

Gives selective brilliant orange fluorescent. Temporary and last for 2 hrs once it is mounted

Answer 16

Fluorescent Acridine Orange Technique