Special Stains (FATS or LIPIDS) Flashcards
Stains LIPIDS and the most sensitive of the oil-soluble dyes
Sudan Black B
Used for NEUTRAL LIPIDS and TRIGLYCERIDES staining them RED and also known as SCHARLACH
Sudan IV
Sudan IV also knows as
Scharlach
First sudan dye; good fat stain for CNS tissue
Sudan III
Nile Blue Stains the following
Neutral Fats
Cholestrin Esters
Cholestrin Fatty Acids
Cerebrosides
Fatty acids
Soap
Stain fats
Oil Red O
Stains Unsaturated fats
Osmic Acid Stain
Stain gangliosides
Borohydride Periodic Schiff
Stains Cholesterol
Perchloric acid-napthoquinone
This acid Intensified and preserve better when added Sudan IV
Benzoic Acid
Unstable oxide used an fixative for EM and Histochemistry
Osmic Acid
Differentiates lipids by its oxazone
RED = Neutral Lipids
BLUE = Basic Lipids
Nile Blue SULFATE
Aka Bodian and Lake
Toluidine Blue