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PHT > Staining > Flashcards

Staining Flashcards

1
Q

Staining conn tissue and cytoplasm

Background stain

Counterstain

A

Eosin

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2
Q

Eosin types

A 
Eosin Y (yellowish) - common
Bluish (Eosin B, Erythrosin B)
Ethyl Eosin (Eosin S, Eosin OH soluble)
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3
Q

Useful as progressive stain

Nuclear stain

Demonstrate cytoplasmic glycogen

Used when acid-OH diff might destroy/decolorize cytoplasmic comp

Used in Celestine Blue hemalum mtd nuclear staining

A

Mayer’s hematoxylin

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4
Q

For diff or regressice staining

Use acid OH as diff agent

A

Iron hematoxylin soln

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5
Q

Diff of muscle fiber and conn tissue

Standard hematoxylin

Uses ferric Ammonium

A

Weigert’s soln

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6
Q

Uses ferric ammonium sulfate

A

Heidenhan’s soln

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7
Q

Mordant: 1% aqueous phosphotungatic acid

Paraffin, celloidin, frozen sections

A

PTAH Phosphotungstic acid hematoxylin

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8
Q

Red
Eosin Y B
Phioxine B

A

Cytoplasmic stain

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9
Q

Yellow
Picric acid
Orange G
Rose bengal

A

Cytoplasmic stain

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10
Q

Green
Light green sf
Lissamine green

A

Cytoplasmic stain

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11
Q 
Red
Neutral red
Safranin O
Hematoxylin
Carmine
A

Nuclear stain

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12
Q

Toluidine blue
Nile blue
Celestine blue

A

Nuclear stain

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13
Q

Gold chloride
Silver nitrate

As precipitates and not absorbed

A

Metallic stain

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14
Q

Saffron
Hematoxylin
Orcein
Cochineal

A

Natural dye

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15
Q

Basic dye

Thizine and triphenylmethane group

A

Metachromatic dye

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16
Q 
Methyl violet
Crystal violet
Cresyl blue retics
Bismarck brown
Safranin
Basic fushin
Toluidine blue
Thionine
Azure a b c
Methylene blue
A

Metachromatic stains

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17
Q

Use of specific dyes

Diff particular subs

Metachromasia

For cartillage, conn tissue, eph mucis, mast cells, amyloid

A

Metachromatic staining

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18
Q

For conn tissue

A

Acid fuschin-picric acid

van gieson stain

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19
Q

Stains collagen, smooth muscle, mitochondria

Nuclear and cytoplasmic stain in mallory’s trichrome mtd

A

Acid fushin (masson stain)

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20
Q

Most commonly used supravital stain

A

Neutral red

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21
Q

For mitochondria

A

Janus green

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22
Q

For dead cells

A

Trypan blue

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23
Q

Toluidine blue
Thionine
Nile blue

A

Supravital (nucleus)

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24
Q

Carmine
Lithium
India ink

A

Intravital

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25
Q

Anilline or coal tar dyes

A

Synthetic dye

26
Q

Discrimination bw dead and living cells

DNA Green fluorescence
RNA Red

A

Acridine orange

27
Q

Demonstrates Ca salts and possible site of phospatase act

A

Acridine Red 3B

28
Q

Stains acid mucopolysaccharides

Excellent striking blue color

A

Alcian blue

29
Q

Orange red lake w/Ca at pH4.2

Used on Dupont ACA analyzer measure serum Ca

A

Alizarin Red S

30
Q

Counterstaining eph sections

A

Aniline blue

31
Q

Plasma stain

For AFOrganism, mitochondria, diff of smooth muscle w/picric acid

Main constituent of Feulgen’s acid and Schiff’s rgt - aldehydes

A

Basic fuschin

32
Q

For conn tissue, mucin, elastic tissue

A

Van gieson’s soln

33
Q

For staining hgb

A

Benzidine

34
Q

Contrast stain for gram’s tech
In acid fast
In pap mtd
For diptheria organism

A

Bismarck brown

35
Q

For fresh materials in smear prep

Det presence of metal ions eg Al

A

Carmine

36
Q

Mordanted dye

Acts as basic dye and stains acid subs

A

Carmalum (mayer’s soln)

37
Q

Alt to iron hematox

A

Celestine blue

38
Q

Best known as indicator

Stain for axis cylinders in embryos

Identify chon depo (amyloid)

A

Congo red

39
Q

In histo for nervous tissue

A

Cresyl violet

40
Q

Stains amyloid in frozen sections and plts

A

Crystal violet

41
Q

For metallic impregnation

A

Gold sublimate

42
Q

Oldest stain

A

Iodine

43
Q

Identify and diff bacteria

A

Gram’s iodine

44
Q

Decolorizer
Counterstain

Weakly basic dye
Contrast stain for ascaris eggs and rbc

Bacterial spore stain

A

Malachite green

45
Q

Formed by boiling nile blue w/sulfuric acid

For living cells

Accumulates in lipid globules inside cells - red

A

Nile red (nile blue oxazone)

46
Q

More soluble in fat than H2O or OH

For neutral lipids

A

Oil red O

47
Q

For elastic fibers

In derma studies

Finest and most delicate skin fiber demonstration

A

Orcein

48
Q

Selective stain for unsaturated lipids
For lipoCHONs
Myelin=black

A 
Osmic acid
Osmium tetroxide (OsO4)
49
Q 
All around stain
Glycogen
Mucin
MucoCHON
GlyCHON
Basement memb
Capsules
Blood vessels
Fungi
Intra carbs glycogen in hepatocytes
A

Periodic acid schiff

50
Q

Common neg stain for viruses, nerves, polysaccharides

For striated muscle fibers
Mitochondria = blue

A

Phosphotungstic acid

51
Q 
Contrast stain to acid fuschin
Cytoplasmic stain
Counterstain to crystal violet
Tissue fixative
Decalcifying agent
A

Picrid acid

52
Q

Used w/ osmic acid to fix and stain blood and glandular tissues

A

Rhodamine B

53
Q

Most senstive

Greater affinity to phospholipids than others lysochromes

A

Sudan black

54
Q

Not real dyes

Do not have auxochrome groups

A

Lysochromes (Oil soluble dyes)

55
Q

No 2ndary amino grp
Does not color phospholipids
For TAG

A

Sudan IV

56
Q

First introduced
Fat soluble
Good fat stain for CNS

A

Sudan III

57
Q

Binds collagen in EM = pink

A

Van gieson stain

58
Q

For neuroglia in frozen section

A

Victoria blue

59
Q

Silver reduction mtd
For phosphates and carbonates
Usually present along Ca

A

Von kossa stain

60
Q

Causes blood cells to exhibit 4 major staining properties

A

Wright stain

PHT (9 decks)

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