Dehydration

Question 1

utilized in plant and animal micro-techniques; slow

dehydrating agent, produces less shrinkage and hardening.

Answer 1

Butyl alcohol

Question 2

Considered to be the best dehydrating agent because it is fast-acting.

Answer 2

Ethyl alcohol

Question 3

toxic dehydrating agent; used for blood and tissue films

and for smear preparations

Answer 3

Methyl alcohol

Question 4

It both dehydrates and clears tissues since it is miscible I water and paraffin.
It may be used for demixing, clearing and dehydrating paraffin sections before and after staining.
It causes less shrinkage and easier cutting of sections with fewer artefacts.
It does not dissolve aniline dyes
It is toxic if ingested or inhaled

Answer 4

Tetrahydrofuran

Question 5

The tissue may be transferred from water or normal saline directly to cellosolve and stored in it for months without producing hardening or distortion.

Propylene based glycol ether should be used instead.

Answer 5

Cellosolve (Ethylene Glycol Monoethyl Ether)

Question 6

Excellent dehydrating and clearing agent

Tissue sections tend to ribbon poorly

Answer 6

Dioxane (Diethylene Dioxide)

Question 7

Cheap, rapid acting dehydrating agent. Dehydrates in ½ to 2 hours.

Answer 7

Acetone

Question 8

It removes water very readily and produces very little distortion and hardening of tissue

Answer 8

Triethyl Phosphate