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PHT > Immunocytohistochemistry > Flashcards

Immunocytohistochemistry Flashcards

1
Q

Produced by immunizing an animal with a purified specific molecule (immunogen) that contains the antigen of interest,
collecting Ig-rich serum after the animal produces humoral antibodies.

A

Polyclonal antibodies

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2
Q

Products of an individual clone of plasma cells

Pruduced through hybridoma and cloning techniques

A

Monoclonal antibodies

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3
Q

absolute methanol, or acetone
to preserve immunological activity
prevent destruction of some labile antigenic site

A

Cryostat section

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4
Q

immuno flluorescence

immunoperoxidase techniques

A

Formaldehyde-fixed and paraffin embedded tissues

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5
Q

highly sensitive, present in epithelial tumors (carcinoma)

A

Keratin

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6
Q

carcinomas of lung, breast, uterus, and ovaries (serous tumors)

A

CK7 (Cytokeratin 7

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7
Q

carcinomas of colon and stomach

A

CK20

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8
Q

Transitional Cell carcinomas of the bladder and mucinous ovarian tumors

A

CK7 and CK20 –

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9
Q

high molecular weight protein that is helpful in determining the site of tumor.
It is positive for adenocarcinomas of the breast, lungs, and kidneys

A

Epithelial Membrane Antigen (EMA)

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10
Q

oncofetal antigen present in carcinomas of the GIT, pancreas, lung, breast, ovary, uterus, and cervix.
It is useful for differentiating adenocarcinoma (+) and mesothelioma (-)

A

Carcinoembryonic Antigen (CEA)

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11
Q

useful in distinguishing lung adenocarcinomas from mesotheliomas

A

Thyroid Transcription Factor-1 (TTF-1)

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12
Q

useful in the diagnosis of prostatic adenocarcinoma

A

Prostate-Specific Antigen (PSA)

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13
Q

sensitive marker for muscle differentiation and can be used to identify tumors derived from muscles

A

Actin

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14
Q

a 57kD intermediate filament that is present in normal mesenchymal cells and their neoplastic counterparts
melanomas and schwannomas stain(+)

A

Vimentin

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15
Q

a 53kD intermediate filament expressed by smooth and striated muscles. It is highly specific for myogenic tumors.

A

Desmin

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16
Q

a 51kD intermediate filament protein expressed by CNS glial cells, particularly astrocytes

A

Glial fibrillary acidic protein (GFAP)

17
Q

expressed in cells of neural origin, particularly neurons, neuronal processes, peripheral nerves, sympathetic ganglia, adrenal medulla, and neuroendocrine cells

A

Neurofilament (NF)

18
Q

low-molecular weight calcium-binding protein that is expressed in CNS glial cells, Schwann cells, melanocytes, histiocytes, chondrocytes, skeletal and cardiac muscle, myoepithelial cells, and some epithelial cells of breast, salivary, and sweat glands

A

S100 Protein

19
Q

provides strong evidence of neural or neuroendocrine differentiation
2.

A

Neuron-specific enolase (NSE)

20
Q

a marker for neuroendocrine differentiation

A

Chromogranin

21
Q

a 38kD transmembrane protein associated with presynaptic vesicles of neurons

A

Synaptophysin

22
Q

synthesized by placental syncytiotrophoblasts

a marker for choriocarcinoma

A

Human Chorionic Gonadotropin (HCG)

23
Q

marker for endodermal sinus tumors showing yolk sac differentiation

A

Alpha-fetoprotein (AFP)

24
Q

marker for germ cell tumors, particularly germinomas

A

Placenta-like alkaline phosphatase (PLAP)

25
Q

positive for muscle-specific actin, desmin, myo D1, myoglobin, and myogenin

A

Myogenic Tumors

26
Q

CD 68, FAM 56, alpha-1-antitrypsin, alpha-1-antichymotrypsin

A

Fibrohistiocytic tumors

27
Q

Factor VII-related antigen, CD 31, Ulex europaeus 1 (UEA)

A

Vascular Tumors

28
Q

S100 protein, melanosome (HMB-45), Melan-A (MART-1)

A

Melanomas

29
Q

– Leukocyte Common Antigen (LCA) or also called CD 45.

A

Lymphomas

30
Q
  • T cell : CD3, CD4, CD8
  • B cells: CD19, CD20, CD23
  • Reed-Sternberg cells: CD15, CD30,
  • Ig light and heavy chains
A

Immunophenotypic, subclass

31
Q

enzyme label
INSOLUBLE DARK BROWN REACTION
Optimal incubation time =

A

Horseradish peroxidase + Diaminobenzidine

30-60 minutes @ room temp.

32
Q

most common immunoglobulin

A

IgG

33
Q

most common animal for monoclonal Ab prod

A

mice

34
Q

most common animal for polyclonal Ab prod

A

rabbit

35
Q

Most common enzyme label

A

peroxidase

36
Q

DAM (diaminobenzidine)

A

brown

37
Q

AEC (aminoethylcarbazole)

A

red

PHT (9 decks)

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