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Histopath, Lec > Staining > Flashcards

Staining Flashcards

1
Q

is the process of applying dyes on the sections to see and study the physical characteristics of the cell

A

staining

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2
Q

process whereby tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution

A

histological staining

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3
Q

various constituents of tissues are studied through chemical reactions that will permit microscopic localization

A

histochemical staining

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4
Q

a combination of immunological and histochemical techniques that allow phenotypic markers

A

immunohistochemical staining

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5
Q

3 major groups of staining

A

histological, histochemical, and immunohistochemical staining

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6
Q

methods of staining

A

direct, indirect, progressive, regressive, differentiation, metachromatic, counterstaining, metallic impregnation and vital staining

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7
Q

a process of giving color to sections by using aqueous or alcoholic dye solution

A

direct staining

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8
Q

a process whereby the action of the dye is intensified by adding another agent

A

indirect staining

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9
Q

serves as a link between tissue and the dye

A

mordant

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10
Q

does not participate in the staining reaction, but merely accelerates or hastens the speed of the staining reaction

A

accentuator

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11
Q

a process whereby tissue elements are stained in definite sequence

A

progressive staining

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12
Q

tissue is overstained to obliterate the cellular details, and the excess stain is removed

A

regressive staining

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13
Q

slower staining process in which the tissue is left in the staining solution

A

progressive

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14
Q

more rapid staining process in which the tissue is deliberately over-stained

A

regressive

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15
Q

usually done by washing the section in simple solution

A

differentiation

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16
Q

two dyes used in gram staining

A

crystal violet and fuchsin or safranin

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17
Q

entails the use of specific dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself

A

metachromatic

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18
Q

application of different color or stain to provide contrast and background

A

counterstaining

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19
Q

process where specific tissue elements are demonstrated, not by stains, but by colorless solutions

A

metallic impregnation

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20
Q

selective staining of living cell constituents

A

vital staining

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21
Q

staining of living cell is done by injecting the dye into any part of animal body

A

intravital staining

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22
Q

used to stain living cells immediately after removal from the living body

A

supravital staining

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23
Q

best vital stain

A

neutral red

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24
Q

recommended for mitochondria

A

janus green

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25
Q

1 g of dye is dissolved in 100ml of sterile dis.

A

trypan blue

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26
Q

nuclei

A

blue black

27
Q

karyosome

A

dark blue

28
Q

cytoplasm

A

pale pink

29
Q

rbc

A

bright orange-red

30
Q

basophil

A

purplish pink

31
Q

cartilage

A

dark blue

32
Q

calcium

A

purplish blue

33
Q

decalcified bone matrix

A

deep pink

34
Q

collagen

A

light pink

35
Q

muscle fibers

A

deep pink

36
Q

most common method utilized for microanatomical studies of tissues

A

routine h&e

37
Q

using regressive staining which consists overstaining the nuclei

A

routine h&e

38
Q

mounted on the slides may be stained as in paraffin sections

A

frozen section staining

39
Q

process where the slide is coated with dilute

A

collodionization of sections

40
Q

old, breached or faded sections may be re-stained

A

re-staining of old sections

41
Q

universal stain for cytological preparations

A

papaniculaou stain

42
Q

used for wet fixed slides

A

romanowsky stain

43
Q

modified ziehl neelsen

A

acid fast bacilli

44
Q

gram staining

A

bacteria

45
Q

mucicarmine

A

mucins

46
Q

periodic acid shiff

A

glycogen

47
Q

oil red o

A

lipids

48
Q

perls prussian blue

A

iron

49
Q

modified fouchet’s test

A

bilirubin

50
Q

the ideal fixative recommended in most of the laboratories

A

95% ethyl alcohol

51
Q

this fixative was originally recommended by papaniculaou

A

ether alcohol mixture

52
Q

acceptable subs for 95 ethanol

A

100 methanol

53
Q

cause slightly more cell shrinkage than ether-ethanol

A

80 propanol and isopropanol

54
Q

it is ethanol that has been changed by the addition of additives in order to render it unsuitable for human consumption

A

denatured alcohol

55
Q

subs for wet fixatives

A

coating fixative

56
Q

special purpose fixative for hemorrhagic samples

A

carnoy’s fixative

57
Q

ideal fixative used for cellblock prep

A

aaf fixative

58
Q

fixed in 95% ethyl alcohol

A

fixation

59
Q

harris hematoxylin

A

nuclear staining

60
Q

OG-6 and EA-36

A

cytoplasmic staining

61
Q

rinse the smears

A

dehydration

62
Q

alcohol is being replaced with xylene

A

clearing

63
Q

mounting media must be miscible with the clearing agent

A

mounting of slide

Histopath, Lec (6 decks)

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