Staining

Question 1

is the process of applying dyes on the sections to see and study the physical characteristics of the cell

Answer 1

staining

Question 2

process whereby tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution

Answer 2

histological staining

Question 3

various constituents of tissues are studied through chemical reactions that will permit microscopic localization

Answer 3

histochemical staining

Question 4

a combination of immunological and histochemical techniques that allow phenotypic markers

Answer 4

immunohistochemical staining

Question 5

3 major groups of staining

Answer 5

histological, histochemical, and immunohistochemical staining

Question 6

methods of staining

Answer 6

direct, indirect, progressive, regressive, differentiation, metachromatic, counterstaining, metallic impregnation and vital staining

Question 7

a process of giving color to sections by using aqueous or alcoholic dye solution

Answer 7

direct staining

Question 8

a process whereby the action of the dye is intensified by adding another agent

Answer 8

indirect staining

Question 9

serves as a link between tissue and the dye

Answer 9

mordant

Question 10

does not participate in the staining reaction, but merely accelerates or hastens the speed of the staining reaction

Answer 10

accentuator

Question 11

a process whereby tissue elements are stained in definite sequence

Answer 11

progressive staining

Question 12

tissue is overstained to obliterate the cellular details, and the excess stain is removed

Answer 12

regressive staining

Question 13

slower staining process in which the tissue is left in the staining solution

Answer 13

progressive

Question 14

more rapid staining process in which the tissue is deliberately over-stained

Answer 14

regressive

Question 15

usually done by washing the section in simple solution

Answer 15

differentiation

Question 16

two dyes used in gram staining

Answer 16

crystal violet and fuchsin or safranin

Question 17

entails the use of specific dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself

Answer 17

metachromatic

Question 18

application of different color or stain to provide contrast and background

Answer 18

counterstaining

Question 19

process where specific tissue elements are demonstrated, not by stains, but by colorless solutions

Answer 19

metallic impregnation

Question 20

selective staining of living cell constituents

Answer 20

vital staining

Question 21

staining of living cell is done by injecting the dye into any part of animal body

Answer 21

intravital staining

Question 22

used to stain living cells immediately after removal from the living body

Answer 22

supravital staining

Question 23

best vital stain

Answer 23

neutral red

Question 24

recommended for mitochondria

Answer 24

janus green

Question 25

1 g of dye is dissolved in 100ml of sterile dis.

Answer 25

trypan blue

Question 26

nuclei

Answer 26

blue black

Question 27

karyosome

Answer 27

dark blue

Question 28

cytoplasm

Answer 28

pale pink

Question 29

rbc

Answer 29

bright orange-red

Question 30

basophil

Answer 30

purplish pink

Question 31

cartilage

Answer 31

dark blue

Question 32

calcium

Answer 32

purplish blue

Question 33

decalcified bone matrix

Answer 33

deep pink

Question 34

collagen

Answer 34

light pink

Question 35

muscle fibers

Answer 35

deep pink

Question 36

most common method utilized for microanatomical studies of tissues

Answer 36

routine h&e

Question 37

using regressive staining which consists overstaining the nuclei

Answer 37

routine h&e

Question 38

mounted on the slides may be stained as in paraffin sections

Answer 38

frozen section staining

Question 39

process where the slide is coated with dilute

Answer 39

collodionization of sections

Question 40

old, breached or faded sections may be re-stained

Answer 40

re-staining of old sections

Question 41

universal stain for cytological preparations

Answer 41

papaniculaou stain

Question 42

used for wet fixed slides

Answer 42

romanowsky stain

Question 43

modified ziehl neelsen

Answer 43

acid fast bacilli

Question 44

gram staining

Answer 44

bacteria

Question 45

mucicarmine

Answer 45

mucins

Question 46

periodic acid shiff

Answer 46

glycogen

Question 47

oil red o

Answer 47

lipids

Question 48

perls prussian blue

Answer 48

iron

Question 49

modified fouchet's test

Answer 49

bilirubin

Question 50

the ideal fixative recommended in most of the laboratories

Answer 50

95% ethyl alcohol

Question 51

this fixative was originally recommended by papaniculaou

Answer 51

ether alcohol mixture

Question 52

acceptable subs for 95 ethanol

Answer 52

100 methanol

Question 53

cause slightly more cell shrinkage than ether-ethanol

Answer 53

80 propanol and isopropanol

Question 54

it is ethanol that has been changed by the addition of additives in order to render it unsuitable for human consumption

Answer 54

denatured alcohol

Question 55

subs for wet fixatives

Answer 55

coating fixative

Question 56

special purpose fixative for hemorrhagic samples

Answer 56

carnoy's fixative

Question 57

ideal fixative used for cellblock prep

Answer 57

aaf fixative

Question 58

fixed in 95% ethyl alcohol

Answer 58

fixation

Question 59

harris hematoxylin

Answer 59

nuclear staining

Question 60

OG-6 and EA-36

Answer 60

cytoplasmic staining

Question 61

rinse the smears

Answer 61

dehydration

Question 62

alcohol is being replaced with xylene

Answer 62

clearing

Question 63

mounting media must be miscible with the clearing agent

Answer 63

mounting of slide