Decalcification Flashcards
done after fixation and before impregnation
decalcification
in surface decalcification, dace down on a pad of cotton saturated with _______ hydrochloric acid for approximately one hour
10%
in surface decalcification, dace down on a pad of cotton saturated with 10% hydrochloric acid for approximately _________
1 hour
four main types of decalcifying agents
acids, chelating agents, ion exchange resins and electrical ionization
most widely used agents for routine decalcification of large amounts of bony tissues because they are stable
acid decalcifying agents
the most common and fastest decalcifying agent
nitric acid
aqueous nitric acid solution 10% time
12-24 hours
formol-nitric acid time
1-3 days
perenyi’s fluid time
2-7 days
phloroglucin-nitric acid time
12-24 hours
most rapid decalcifying agent so far
phloroglucin-nitric acid
inferior compared to nitric acid in its role
hydrochloric acid
von ebner’s fluid time:
3-5 days
moderate-acting decalcifying agent
formic accid
aqueous formic acid time
2-7 days
formic acid-sodium citrate solution time
3-14 days
trichloroacetic acid time
4-8 days
it permits good nuclear staining
trichloroacetic acid
a very weak decalcifying solution suitable only for minute pieces of bone
sulfurous acid
chromic acid (flemming’s fluid)
12 hours
can be both used as a fixative and decalcifying agent
chromic acid (flemming’s fluid)
an environmental toxin
chromic acid
citric acid-citrate buffer solution
6 days
ph of citric acid-citrate buffer solution
ph 4.5
